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Callus Induction and Plant Regeneration in Chinese Kale

机译：中国羽衣甘蓝的愈伤组织诱导和植物再生

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摘要

Using the cotyledon and hypocotyl of Chinese kale 'Sijicutiao' as explants, the effects of MS medium supplemented with different types and concentrations of growth regulators (2, 4-D, 6-BA, NAA) on the callus induction and regeneration of Chinese kale were studied. The results showed that different hormone combinations of 2,4-D, 6-BA and NAA play a key role in the callus induction and regeneration culture of Chinese kale, and hypocotyl has a better effect than cotyledon with petiole as an explant. MS + 0.1 mgL-12, 4-D + 0.02 mgL-1 6-BA was the optimal combination in the process of induction, the hypocotyl explant induction rate was 80%. During the regeneration culture, the best combination was MS + 0.75 mgL-1 6-BA + 0.03 mgL-1 NAA, the differentiation rate of adventitious buds was 94%, and the rooting rate was 67%. These results could provide a theoretical reference value for the study of cell suspension culture of Chinese kale.
机译：使用中国羽衣甘蓝的子叶和胚乳作为外植体，补充了不同类型和生长调节剂（2,4-D，6-BA，NAA）的MS培养基对中国羽衣甘蓝的愈伤组织诱导和再生的影响研究过。结果表明，不同的激素组合为2,4-D，6-BA和NAA在中国羽衣甘蓝的愈伤组织诱导和再生培养中发挥关键作用，并且缺口基于与叶柄作为外植体的子叶具有更好的效果。 MS + 0.1MgL-12，4-D + 0.02 MgL-1 6-BA是诱导过程中的最佳组合，缺口植体诱导率为80％。在再生培养过程中，最佳组合是MS + 0.75mgL-1 6-BA + 0.03mg-1 NAA，不定芽的分化率为94％，生根率为67％。这些结果可以为中国羽衣甘蓝细胞悬浮培养的研究提供理论参考价值。

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