Cloning and Sequence Analysis of BjuCYP1 Gene in Brassica Juncea

机译：Brassica Juncea的Bjucyp1基因的克隆与序列分析

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CYP gene is a member of the cytochrome P450 superfamily, which plays an important role in plant growth, signals transduction and defense responsive. The BjuCYPl gene of Brassica juncea was cloned, and then bioinformatics of this gene was analyzed. The results showed that the gene contained a 516 bp complete open reading frame and encoded a protein of 171 amino acids, with a molecular weight of 18.09 kD and a theoretical isoelectric point (pI) of 8.66. Homologous sequence alignment revealed that BjuCYPl was closely related to CYP proteins of turnip and radish, which all belonged to Cruciferous. Subcellular localization predicted that the protein was located in the cytoplasm. This study provides useful information to explore the molecular function of BjuCYPl in B. juncea.

机译：CYP基因是细胞色素P450超家族的成员，其在植物生长中起重要作用，信号转导和防御响应性。克隆了Brassica Juncea的BjucyPL基因，然后分析了该基因的生物信息学。结果表明，该基因含有516bp完全开放阅读框架，并编码171个氨基酸的蛋白质，分子量为18.09kd和8.66的理论等电点（pi）。同源序列对准显示，BjucyP1与萝卜和萝卜的CYP蛋白密切相关，萝卜均属于白霉素。亚细胞定位预测蛋白质位于细胞质中。本研究提供了有用的信息，以探讨B. juncea的Bjucypl的分子功能。

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《International Conference on Frontiers of Biological Sciences and Engineering》|2020年|1 volume (various pagings) :|共5页
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Fangjie Xie; Shanshan Tan; Jie Li; Mengyao Li;
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中图分类 Q81-532;
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Brassica juncea; BjuCYPl; Cloning; Sequence Analysis;

机译：Brassica Juncea;Bjucypl;克隆;序列分析;

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Cloning and Sequence Analysis of BjuCYP1 Gene in Brassica Juncea

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