HoxU of Synechocystis sp. PCC 6803 Heterologous Expressed in E. Aerogenes

机译：Synechocystis sp的Hoxu。 PCC 6803异源在E. Ailogenes中表达

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In this study, hydrogenase gene hoxU of the Cyanobacteria Synechocystis sp. PCC 6803 was cloned and heterologous expressed in Enterobacter aerogenes by using the pGEX-4T-2-cat vector, and obtain the combinant strain. The hydrogen yield of recombinant strain has increased compared with the wild type. The hydrogen yield of recombinant strain E. aerogenes/HoxU was promoted from 1.14 mol H2/mol glucose to 2.12 mol H2/mol glucose and the peak hydrogen producing rate reached 83.5 mL.L-1.h-1, which was higher than that of wild type.

机译：在本研究中，豆蔻酸型鞘膜细胞Sp的氢酶基因Hoxu。通过使用PGEX-4T-2-CAT载体，在肠杆菌空气中克隆和异源克隆和异源，得到组合菌株。与野生型相比，重组菌株的氢产率增加。从1.14mol H 2 / Mol葡萄糖促进重组菌株E. Aerogens / Hoxu的氢产率至2.12molH2 / mol葡萄糖，达到83.5ml-1.h-1的峰值氢气产生率高于此野生型。

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《International Conference on Frontiers of Biological Sciences and Engineering》|2020年|1 volume (various pagings) :|共4页
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Junyan Zhu; Hui Chu; Huanmei Li; Wa Gao; Jinfang Zhao;
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中图分类 Q81-532;
关键词
heterologous expressed; Enterobacter aerogenes; using;

机译：异源表达;肠杆菌空气;使用;

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1. 蓝细菌Synechocystis sp.PCC6803中叶绿素合成调控类囊体膜重建的研究 [J] . 吴庆余, 徐红植物学报：英文版 . 1997,第011期
2. Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803 Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803 Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803 [J] . Vita Vidmar, Aneja Tahirovi?, Marko Dolinar, Biology Open . 2016,第4期

机译：生物技术上重要的微藻的生物安全性：蓝藻Synechocystis sp。中固有的自杀开关实施。 PCC 6803生物技术上重要的微藻的生物安全性：蓝藻集胞藻属中固有的自杀开关实施。 PCC 6803生物技术上重要的微藻的生物安全性：蓝藻集胞藻属中固有的自杀开关实施。 PCC 6803
3. Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803 Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803 Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803 [J] . Vita Vidmar, Aneja Tahirovi?, Marko Dolinar, Biology Open . 2016,第4期

机译：生物技术上重要的微藻的生物安全性：蓝藻Synechocystis sp。中固有的自杀开关实施。 PCC 6803生物技术上重要的微藻的生物安全性：蓝藻集胞藻属中固有的自杀开关实施。 PCC 6803生物技术上重要的微藻的生物安全性：蓝藻集胞藻属中固有的自杀开关实施。 PCC 6803
4. Attachment of Noncognate Chromophores to CpcA of Synechocystis sp. PCC 6803 and Synechococcus sp. PCC 7002 by Heterologous Expression in Escherichia coli [J] . Richard M. Alvey Avijit Biswas Wendy M. Schluchter and Donald A. Bryant Biochemistry . 2011,第22期

机译：非同源发色团附着到集胞藻的CpcA。 PCC 6803和Synechococcus sp。 PCC 7002在大肠杆菌中的异源表达
5. HoxU of Synechocystis sp. PCC 6803 Heterologous Expressed in E. Aerogenes [C] . Junyan Zhu, Hui Chu, Huanmei Li, International Conference on Frontiers of Biological Sciences and Engineering . 2020

机译：Synechocystis sp的Hoxu。 PCC 6803异源在E. Ailogenes中表达
6. In vitro investigations of the hemoglobins from the bacteria Synechocystis sp. PCC 6803, Synechococcus sp. PCC 7002, and Helicobacter hepaticus. [D] . Nothnagel, Henry J. 2010

机译：细菌Synechocystis sp。的血红蛋白的体外研究。 PCC 6803，Synechococcus sp。 PCC 7002和肝幽门螺杆菌。
7. Improving heterologous protein expression in Synechocystis sp. PCC 6803 for alpha-bisabolene production [O] . Jacob Sebesta, Christie AM. Peebles 2020

机译：改善异胞藻属中的异源蛋白表达。 PCC 6803用于生产α-双萜烯
8. Growth arrest of Synechocystis sp. PCC6803 by superoxide generated from heterologously expressed Rhodobacter sphaeroides chlorophyllide a reductase [O] . Kim Eui-Jin, Kim Ju-Sim, Rhee Hae Jin, 2009

机译：蓝藻的生长停滞。 PCC6803由超氧化物生成的异源表达的球形红球菌叶绿素一还原酶
9. Heterologous Assembly and Rescue of Stranded Phycocyanin Subunits by Expressionof a Foreign cpcBA Operon in Synechocystis sp. Strain 6803 [R] . Plank, T., Anderson, L. K. 1995

机译：通过在集胞藻中表达外源cpcBa操纵子来异源组装和拯救链状藻蓝蛋白亚基。应变6803

HoxU of Synechocystis sp. PCC 6803 Heterologous Expressed in E. Aerogenes

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