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PROTEOMIC ANALYSIS OF HAIR FOLLICLES

机译：毛囊蛋白质组学分析

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Hair root cells actively divide in a hair follicle, and they sensitively reflect physical conditions. By analyzing the human hair, we can know stress levels on the human body and metabolic conditions caused by microgravity environment and cosmic radiation. The Japan Aerospace Exploration Agency (JAXA) has initiated a human research study to investigate the effects of long-term space flight on gene expression and mineral metabolism by analyzing hair samples of astronauts who stayed in the International Space Station (ISS) for 6 months. During long-term flights, the physiological effects on astronauts include muscle atrophy and bone calcium loss. Furthermore, radiation and psychological effects are important issue to consider. Therefore, an understanding of the effects of the space environment is important for developing countermeasures against the effects experienced by astronauts. In this experiment, we identify functionally important target proteins that integrate transcriptome, mineral metabolism and proteome profiles from human hair. To compare the protein expression data with the gene expression data from hair roots, we developed the protein processing method. We extracted the protein from five strands of hair using ISOGEN reagents. Then, these extracted proteins were analyzed by LC-MS/MS. These collected profiles will give us useful physiological information to examine the effect of space flight.
机译：头发根细胞在毛囊中积极分开，它们敏感地反映了物理条件。通过分析人的头发，我们可以了解人体的压力水平和由微匍匐环境和宇宙辐射引起的人体和代谢条件。日本航空航天勘探机构（JAXA）已启动人类研究，以调查长期空间飞行对基因表达和矿物代谢的影响，分析宇航员的宇航员的头发样本，持续在国际空间站（ISS）持续6个月。在长期航班期间，对宇航员的生理效应包括肌肉萎缩和骨钙损失。此外，辐射和心理效应是需要考虑的重要问题。因此，对空间环境的影响了解对宇航员经历的效果的对策非常重要。在该实验中，我们鉴定了功能性重要的靶蛋白，其将转录组，矿物代谢和蛋白质组谱系从人发中整合。为了将蛋白质表达数据与来自毛根的基因表达数据进行比较，我们开发了蛋白质加工方法。我们使用异链试剂将蛋白质从五片毛发中提取。然后，通过LC-MS / MS分析这些提取的蛋白质。这些收集的型材将为我们提供有用的生理信息来检查空间飞行的效果。

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