首页> 外文会议>PEGS >Human Synthetic, Semi-Synthetic, and Na?ve Immune Libraries for Efficient Isolation of Antigen Specific Antibodies Isolation of Antigen Specific Antibodies

【24h】

Human Synthetic, Semi-Synthetic, and Na?ve Immune Libraries for Efficient Isolation of Antigen Specific Antibodies Isolation of Antigen Specific Antibodies

机译：人合成，半合成和Na ve've免疫文库，用于有效地分离抗原特异性抗体的分离分离抗原特异性抗体

获取原文

页面导航

摘要
著录项
相似文献
相关主题

摘要

To isolate antigen specific antibodies is critical for a variety of diagnostic, therapeutic, and research purposes. For efficient isolation of antibodies against a target antigen at ease with bypassing animal immunization, we have constructed three kinds of antibody libraries, synthetic, semi-synthetic, and human na?ve immune libraries, composed of a huge number of individual transformants (size: > 10[10-11]) for the display of single chain variable fragment (scFv) antibody variants on the minor coat protein of bacteriophages. To improve the quality of the antibody libraries, both growth based selection and FACS based selection were employed and the utility of the constructed libraries were validated by screening against three model antigens.

机译：分离抗原特异性抗体对于各种诊断，治疗和研究目的至关重要。为了高效地分离抗体以易于绕过动物免疫，我们构建了三种抗体文库，合成，半合成和人类Na-VE免疫文库，由大量的单独转化体组成（大小： > 10 [10-11]）用于显示单链可变片段（SCFV）抗体变体在噬菌体的次要涂层蛋白质上。为了提高抗体文库的质量，采用基于生长的选择和FACS选择，并通过筛选三种模型抗原来验证构建文库的效用。

著录项

来源
《PEGS》|2016年|CD-ROM ;|共1页
会议地点
作者
Man-Seok Ju; Sang Taek Jung;
展开▼
作者单位

展开▼
会议组织
原文格式 PDF
正文语种
中图分类生物工程学（生物技术）;
关键词

相似文献

外文文献
中文文献
专利

1. Use of antibody gene library for the isolation of specific single chain antibodies by ampicillin-antigen conjugates [J] . Neumann-SchaalM., MesserschmidtK., GrenzN., Immunology Letters . 2013,第1a2期

机译：抗体基因文库用于通过氨苄青霉素-抗原结合物分离特定单链抗体的用途
2. In vivo antigen-driven plasmablast enrichment in combination with antigen-specific cell sorting to facilitate the isolation of rare monoclonal antibodies from human B cells [J] . Zhonghua Lin, Nancy Y Chiang, Ning Chai, Nature protocols erecipes for researchers . 2014,第7期

机译：体内抗原驱动的成浆细胞富集与抗原特异性细胞分选相结合，以促进从人B细胞中分离稀有单克隆抗体
3. Isolation of human single chain variable fragment antibodies against specific sperm antigens for immunocontraceptive development. [J] . Samuel AS, Naz RK Human Reproduction . 2008,第6期

机译：针对特定精子抗原的人单链可变片段抗体的分离，用于免疫避孕开发。
4. Human Synthetic, Semi-Synthetic, and Na?ve Immune Libraries for Efficient Isolation of Antigen Specific Antibodies Isolation of Antigen Specific Antibodies [C] . Man-Seok Ju, Sang Taek Jung PEGS . 2016

机译：人合成，半合成和Na ve've免疫文库，用于有效地分离抗原特异性抗体的分离分离抗原特异性抗体
5. Fully human antigen-specific polyclonal antibody responses induced in cloned human artificial chromosome transchromosomic cattle. [D] . Choi, Yoon Jong. 2005

机译：在克隆的人类人工染色体转染色体牛中诱导的完全人类抗原特异性多克隆抗体反应。
6. Isolation of Dengue Virus-Specific Memory B Cells with Live Virus Antigen from Human Subjects following Natural Infection Reveals the Presence of Diverse Novel Functional Groups of Antibody Clones [O] . Scott A. Smith, A. Ruklanthi de Alwis, Nurgun Kose, 2014

机译：自然感染后从人类受试者中分离出带有活病毒抗原的登革热病毒特异性记忆B细胞揭示了抗体克隆新功能基团的存在
7. Isolation of human single chain variable fragment antibodies against specific sperm antigens for immunocontraceptive development [O] . Samuel, A.S., Naz, R.K. 2008

机译：针对特异性精子抗原的人单链可变片段抗体的分离，用于免疫避孕开发
8. In vitro Antigen-Specific Antibody Response to the Species-Specific Surface Protein Antigens of Typhus Group Rickettsiae by Human Peripheral Blood Mononuclear Cells. Generation of an Antigen-Dependent Suppressor T Cell [R] . Misiti, J., Dasch, G. A. 1985

机译：体外抗原特异性抗体对人外周血单个核细胞对斑疹伤寒组立克次体的物种特异性表面蛋白抗原的反应。产生抗原依赖性抑制T细胞

Human Synthetic, Semi-Synthetic, and Na?ve Immune Libraries for Efficient Isolation of Antigen Specific Antibodies Isolation of Antigen Specific Antibodies

摘要

著录项

相似文献

相关主题

期刊订阅