• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Molecular Medicine Tri-Conference. >Lyo-Ready SuperScript Reverse Transcriptases: High-Performance Lyophilization-Compatible Enzymes for RT-PCR-Based Assays
【24h】

Lyo-Ready SuperScript Reverse Transcriptases: High-Performance Lyophilization-Compatible Enzymes for RT-PCR-Based Assays

机译：Lyo-ready上标逆转录酶：高性能冻干 - 基于RT-PCR的测定酶

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nucleic acid detection assays in lyophilized (freeze-dried) formats provide many advantages over conventional wet formats in terms of higher stability and lower shipping costs. Conventional formulations of reverse transcriptases (RTs) used for RT-PCR contain up to 50% glycerol, which serves as a cryoprotectant and stabilizes enzymes for extended periods of time. However, even at very low concentrations such as 0.5%, glycerol can interfere with the lyophilization process. Therefore, most conventional enzyme preparations are incompatible with lyophilization. Thermo Fisher Scientific has developed lyophilization-ready (lyo-ready) enzyme formulations of Invitrogen? SuperScript?IV (SSIV) and SuperScript III (SSIII) RTs that meet high-performance requirements of RT-PCR-based assays. The new lyo-ready RTs are formulated with <0.01% glycerol and retain all inherent characteristics of preparations with glycerol, providing a powerful tool for development of sensitive and reproducible RT-PCR assays in dry formats.
机译：冻干（冷冻干燥）形式中的核酸检测测定在更高的稳定性和较低运费方面提供了与常规湿格式的许多优点。用于RT-PCR的逆转录酶（RTS）的常规制剂含有高达50％的甘油，其用作冷冻保护剂并稳定少时的酶。然而，即使在诸如0.5％的非常低的浓度下，甘油也会干扰冻干过程。因此，大多数常规酶制剂与冻干不相容。 Thermo Fisher Scientific已经开发了冻干准备（Lyo-ready）酶的Invitrogen酶配方？上标？IV（SSIV）和上标III（SSIII）RTS，其满足基于RT-PCR的测定的高性能要求。新的Lyo-Ready RTS配制成<0.01％甘油，并保留与甘油的制剂的所有固有特性，为干燥形式的敏感和可重复的RT-PCR测定提供了强大的工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号