Determining viability of porcine mycoplasma cultures using a redox indicator

机译：使用氧化还原指标确定猪支原体培养的活力

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Mycoplasma hyopneumoniae (Mhp), Mycoplasma hyorhinis (Mhr), and Mycoplasma hyosynoviae (Mhs) have caused a big economic loss in the pig industry worldwide. In order to prevent mycoplasma infection, pure cultures were submitted from university veterinary diagnostic laboratories to federal licensed biological companies for autogenous vaccine production. PCR assays are used to identify mycoplasma species. However, PCR results cannot tell the difference between viable and dead cultures. In order to determine viability of the submitted cultures, a non-toxic redox (oxidation-reduction) indicator (alamarBlue) was used. The alamarBlue (life technologies) assay is designed to monitor the reducing environment of proliferating mycoplasma culture.1 If the tested culture is alive, innate metabolic activity of mycoplasma culture will make alamarBlue in reduced form (pink in color). If the tested culture is dead, alamarBlue will be in the oxidized form (blue in color).

机译：支原体源性氧化物（MHP），支原体症症（MHR）和支原体杂志（MHS）在全球猪工业中造成了巨大的经济损失。为了预防床脊髓瓣感染，纯文化从大学兽医诊断实验室提交到联邦持有的生物公司，用于自动疫苗生产。 PCR测定用于鉴定支原体物种。然而，PCR结果不能讲解可行和死亡培养之间的差异。为了确定所提出的培养物的活力，使用无毒的氧化还原（氧化还原）指标（Alamarblue）。 Alamarblue（Life Technologies）测定旨在监测增殖支原体培养的还原环境.1如果测试的培养物活着，支原体培养的先天代谢活性将以缩小的形式（粉红色）制作艾拉明植物。如果测试的培养物死了，则艾拉明菊属将是氧化形式（蓝色为蓝色）。

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《Annual^Meeting of the American^Association^of^Swine^Veterinarians.》|2016年|434 p. :|共2页
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Boh Chang Lin; JeffKula; Terry Marco;
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1. Cytopathogenicity of Mycoplasma hyopneumoniae in porcine tracheal ring and lung explant organ cultures alone and in combination with monolayer cultures of fetal lung fibroblasts. [J] . P P Williams, J E Gallagher Infection and immunity . 1978,第2期

机译：猪气管环和肺外植体器官培养物中单独和结合胎肺成纤维细胞的单层培养时，猪肺炎支原体的细胞致病性。
2. A Multiplex PCR to Identify Porcine Mycoplasmas Present in Broth Cultures [J] . T. Stakenborg, J. Vicca, P. Butaye, Veterinary Research Communications . 2006,第3期

机译：多重PCR鉴定肉汤培养物中存在的猪支原体
3. Ciliostasis and loss of cilia induced by Mycoplasma hyopneumoniae in porcine tracheal organ cultures. [J] . M C DeBey, R F Ross Infection and immunity . 1994,第12期

机译：猪气管器官培养物中猪肺炎支原体引起的纤毛停滞和纤毛丢失。
4. Determining viability of porcine mycoplasma cultures using a redox indicator [C] . Boh Chang Lin, JeffKula, Terry Marco Annual^Meeting of the American^Association^of^Swine^Veterinarians. . 2016

机译：使用氧化还原指标确定猪支原体培养的活力
5. Genetic study of three porcine mycoplasma species and Mycoplasma fermentans. [D] . Huang, Yiping. 1994

机译：三种猪支原体和发酵支原体的遗传研究。
6. Cytopathogenicity of Mycoplasma hyopneumoniae in porcine tracheal ring and lung explant organ cultures alone and in combination with monolayer cultures of fetal lung fibroblasts. [O] . P P Williams, J E Gallagher 1978

机译：猪肺气管环和肺外植体器官培养物中以及猪胎肺成纤维细胞的单层培养物中结合的猪肺炎支原体的细胞致病性。
7. Pathogenic effects of Mycoplasma hyopneumoniae on porcine tracheal organ cultures and neutrophils in vitro, and porcine respiratory goblet cells in vivo [O] . DeBey, Mary C. 1992

机译：猪肺炎支原体对猪气管器官培养物和中性粒细胞的致病作用以及体外对猪呼吸杯状细胞的致病作用
8. The Quantitative Swab Culture and Smear: A Quick, Simple Method for Determining the Number of Viable Aerobic Bacteria on Open Wounds. [R] . Levine, N. S., Lindberg, R. B., Mason, A. D., 1975

机译：定量拭子培养和涂片：一种快速，简便的方法来确定开放性伤口上活的好氧细菌数量。

Determining viability of porcine mycoplasma cultures using a redox indicator

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