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Multiple Shoots Induction and Rapid Propagation of Lonicera Edulis

机译：多次射击诱导和Lonicera Edulis的快速繁殖

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Sprouts being explants, the factors affecting in vitro culture and plant regeneration of L. edulis were optimized by orthogonal design and tissue cultured seedlings were obtained on culture media with different concentrations of hormones. The results showed that the optimum compositions for primary culture, subculture and rooting culture were MS+6-BA 1.0 mg/L + IBA 0.2 mg/L, MS+6-BA 0.5 mg/L +IBA 0.3 mg/L +KT 1.5 mg/L and 1/4MS+IBA 1.5 mg/L. The differentiation rate, proliferation coefficient and rooting rate on these media were up to 100%, 8.7, and 96.7% respectively. The combination of 6-BA, IBA and KT was effective in the number of multiple shoots of L. edulis in subculture. The micro-propagation technology introduced to cultivate the tissue cultured seedlings proved to meet the need of rapid propagation of plantlets and could be applied in mass propagation.
机译：豆芽是外植体，通过正交设计和组织培养幼苗在具有不同浓度的激素的培养基上获得了影响的体外培养和植物再生的因素。结果表明，初级培养，亚培养和生根培养的最佳组合物是MS + 6-Ba 1.0mg / L + IBA 0.2mg / L，MS + 6-Ba 0.5mg / L + IBA 0.3mg / L + Kt 1.5 Mg / L和1 / 4ms + IBA 1.5 mg / L.这些培养基上的分化率，增殖系数和生根率可达100％，8.7和96.7％。 6-BA，IBA和KT的组合在传代培养的L. Edulis的多个芽的数量中是有效的。引入培养组织培养的幼苗的微传播技术证明了满足植物快速繁殖的需要，并且可以在大规模繁殖中施用。

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