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Multi-spectral endogenous fluorescence imaging for bacterial differentiation

机译:细菌分化的多光谱内源荧光成像

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In this paper, the multi-spectral endogenous fluorescence imaging was implemented for bacterial differentiation. The fluorescence imaging was performed using a digital camera equipped with a set of visual bandpass filters. Narrowband 365 nm ultraviolet radiation passed through a beam homogenizer was used to excite the sample fluorescence. In order to increase a signal-to-noise ratio and suppress a non-fluorescence background in images, the intensity of the UV excitation was modulated using a mechanical chopper. The principal components were introduced for differentiating the samples of bacteria based on the multi-spectral endogenous fluorescence images.
机译:本文实施了多光谱内源荧光成像以进行细菌分化。使用配备有一组视觉带通滤波器的数码相机进行荧光成像。通过光束均化器通过窄带365nm紫外线辐射激发样品荧光。为了增加信噪比并抑制图像中的非荧光背景,使用机械斩波器调制UV激发的强度。引入主要成分用于将基于多光谱内源荧光图像的细菌样品分化。

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