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Dynamic Promotion and Suppression Model for Plasmid Conjugal Transfer Under a Flow Condition

机译:流动条件下质粒蛋白转移的动态促进和抑制模型

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Recipients (the plasmid-free bacteria) receive a copy of plasmid from donors (the plasmid-bearing bacteria) and become transconjugants. In case of the plasmid has a gene providing a drug resistant, recalcitrant substance and so on, these functions are transferred with plasmid carrying. Plasmid transfer makes a substantial contribution an ability to adjust to the environment. Plasmid transfer is mainly performed by conjugation, called conjugal transfer, of a pair of bacteria. Conjugal transfer need to approach the bacteria bodies. Meanwhile, in nature, bacteria lives in not only solid conditions such as soil but also liquid conditions such as rivers. Therefore dynamic analysis of plasmid in the liquid condition is very important. Conjugal transfer is affected by a stream of the liquid condition, because the behavior of bacteria is controlled by the stream. However, relationship between flow condition and the number of transconjugants isn't currently clarified. Therefore, in this study, we aim at analysis of plasmid dynamics under the flow condition. We develop a model with promotion and suppression term for relations between flow condition and the number of transconjugants, and identifying parameters by using the experimental data at agitating flask.
机译:受体(无质粒细菌)接受来自供体(轴承细菌)的质粒拷贝,并成为经杂交剂。在质粒的情况下,具有提供耐药性,醋酸性物质等的基因,这些功能用质粒携带转移。质粒转移对环境进行了大量的贡献。质粒转移主要是通过缀合,称为焦油转移的缀合物,其细菌。蛋白转移需要接近细菌体。同时,本质上,细菌不仅居住在土壤(如土壤)等固体条件下,也有河流等液体条件。因此,在液体条件下对质粒的动态分析非常重要。蛋白转移受液体条件流的影响,因为细菌的行为由流控制。然而,目前澄清了流动条件与经杂交师的数量之间的关系。因此,在本研究中,我们的目的是在流动条件下分析质粒动力学。我们在流动条件和经型烧瓶中使用实验数据识别参数的促销和抑制术语,以及通过在搅拌烧瓶中使用实验数据来开发一个模型。

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