Local structure analysis of heme protein by resonance Raman spectroscopy in the gas phase

机译：气相中共振拉曼光谱血红素蛋白的局部结构分析

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Recent gas-phase spectroscopy has a great advantage for structural analysis of large biomolecules combined with electrospray ionization, where the high-sensitive and high- resolution measurement can be performed based on the stable ionization source. However in huge biomolecules, the conventional IR-UV double resonance spectroscopy cannot be used because of their extremely large degree of vibrational freedom. Therefore, we have developed a novel gas-phase resonance Raman spectroscopy by using IR-laser ablation of droplet beam . This spectroscopy effectively avoids the spectral boarding by observation of the local site near the excited chromophore.

机译：最近的气相光谱对大型生物分子的结构分析具有很大的优势，可以基于稳定的电离源进行高敏感和高分辨率测量。然而，在巨大的生物分子中，由于其极大程度的振动自由度，不能使用常规的IR-UV双共振光谱。因此，我们通过使用IR-Laser消融液滴束开发了一种新的气相共振拉曼光谱。该光谱学通过观察激发的发色团附近的局部位点有效地避免了光谱登机。

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《日本化学会春季年会講演予稿集》|2018年|1 CD-ROM|共1页
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Hiroya Asami; Norishi Kawauchi; Rina Kawabata;
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中图分类石油化学工业;
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1. Solution and crystal phase resonance Raman spectroscopy: Valuable tools to unveil the structure and function of heme proteins [J] . Smulevich Giulietta Journal of porphyrins and phthalocyanines . 2019,第7a8期

机译：溶液和晶相谐振拉曼光谱：揭示血红素蛋白的结构和功能的有价值的工具
2. A Comparative Resonance Raman Analysis of Heme-Binding PAS Domains: Heme Iron Coordination Structures of the BjFixL, AxPDEA1, EcDos, and MtDos Proteins [J] . Takeshi Tomita, Gonzalo Gonzalez, Alan L.Chang, Biochemistry . 2002,第15期

机译：血红素结合PAS域的比较共振拉曼分析：BjFixL，AxPDEA1，EcDos和MtDos蛋白的血红素铁配位结构。
3. Assignment of heme resonances and determination of the electronic structures of high- and low-spin nitrophorin 2 by (1)h and (13)c NMR spectroscopy: an explanation of the order of heme methyl resonances in high-spin ferriheme proteins. [J] . Shokhireva TKh, Shokhirev NV, Walker FA Biochemistry . 2003,第3期

机译：通过（1）h和（13）c NMR光谱确定血红素共振并确定高旋和低旋硝化蛋白2的电子结构：高旋铁蛋白血红素甲基共振顺序的解释。
4. Local structure analysis of heme protein by resonance Raman spectroscopy in the gas phase [C] . Hiroya Asami, Norishi Kawauchi, Rina Kawabata 日本化学会春季年会講演予稿集 . 2018

机译：气相中共振拉曼光谱血红素蛋白的局部结构分析
5. Resonance Raman investigations of heme proteins in the liquid, frozen and crystalline phase: A study of the heme pocket of myoglobin. [D] . Morikis, Dimitrios. 1990

机译：液相，冷冻和结晶相中血红素蛋白的共振拉曼研究：对肌红蛋白血红素袋的研究。
6. Resonance Raman spectroscopy reveals that substrate structure selectively impacts the heme-bound diatomic ligands of CYP17 [O] . Piotr J. Mak, Michael C. Gregory, Stephen G. Sligar, -1

机译：共振拉曼光谱显示底物结构选择性影响CYP17的血红素结合双原子配体
7. A Comparative Resonance Raman Analysis of Heme-Binding PAS Domains: Heme Iron Coordination Structures of theBjFixL,AxPDEA1,EcDos, andMtDos Proteins, [O] . Takeshi Tomita, Gonzalo Gonzalez, Alan L. Chang, 2002

机译：血红素结合域的比较共振拉曼分析：BJFIX1，AXPDEA1，ECDO，ANDMTDOS蛋白的血红素铁协调结构，
8. Axial Coordination in Nickel Porphyrins and Nickel-Reconstituted Heme Proteins Investigated by Raman-Difference and Transient-Raman Spectroscopy. [R] . Shelnutt, J. A., Alston, K., Findsen, E. W., 1986

机译：通过拉曼差和瞬态拉曼光谱研究镍卟啉和镍重构血红素蛋白的轴向配位。

Local structure analysis of heme protein by resonance Raman spectroscopy in the gas phase

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