Construction of a Two-Dimensional Protein Array Utilizing a Cytochrome c555-Based Protein

机译：利用细胞色素C555蛋白的二维蛋白阵列的构建

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We have previously constructed a building block protein (BBP) based on cytochrome c555 circular permutation and α-helix insertion (Figure 1). The X-ray structure of the BBP trimer exhibited a domain-swapped triangle-shaped structure, and four BBP trimers formed a tetrahedron in the crystal.

机译：我们以前构建了基于细胞色素C555圆形置换和α-螺旋插入的构建块蛋白（BBP）（图1）。 BBP三角形的X射线结构表现出畴旋转的三角形结构，并且四个BBP三晶片在晶体中形成了四面体。

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《日本化学会春季年会講演予稿集》|2018年|1 CD-ROM|共1页
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Cheng Xie; Masaru Yamanaka; Satoshi Nagao;
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中图分类石油化学工业;
关键词
Circular permutation; Domain-swapping; Protein array; Protein supramolecule;

机译：循环排列;域交换;蛋白质阵列;蛋白质超分子;

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专利

1. Proteomic analysis of human plasma proteins by two-dimensional gel electrophoresis and by antibody arrays following depletion of high-abundance proteins. [J] . Desrosiers RR, Beaulieu E, Buchanan M, Cell biochemistry and biophysics . 2007,第3期

机译：高血浆蛋白耗尽后，通过二维凝胶电泳和抗体阵列对人类血浆蛋白进行蛋白质组学分析。
2. Proteomic Analysis of Human Plasma Proteins by Two-Dimensional Gel Electrophoresis and by Antibody Arrays Following Depletion of High-Abundance Proteins [J] . Richard R. Desrosiers, Édith Beaulieu, Marguerite Buchanan, Cell Biochemistry and Biophysics . 2007,第3期

机译：人血浆蛋白的蛋白质组学分析，通过二维凝胶电泳和耗尽高丰度蛋白后的抗体阵列进行
3. Mapping and identification of HeLa cell proteins separated by immobilized pH-gradient two-dimensional gel electrophoresis and construction of a two-dimensional polyacrylamide gel electrophoresis database [J] . Allan Christian Shaw, Martin Rossel Larsen, Peter Roepstorff, Electrophoresis: The Official Journal of the International Electrophoresis Society . 1999,第4a5期

机译：固定化pH梯度二维凝胶电泳分离HeLa细胞蛋白的定位和鉴定以及二维聚丙烯酰胺凝胶电泳数据库的建立
4. Construction of a Two-Dimensional Protein Array Utilizing a Cytochrome c555-Based Protein [C] . Cheng Xie, Masaru Yamanaka, Satoshi Nagao 日本化学会春季年会講演予稿集 . 2018

机译：利用细胞色素C555蛋白的二维蛋白阵列的构建
5. Part I---Evaluating Effects of Oligomer Formation on Cytochrome P450 2C9 Electron Transfer and Drug Metabolism, Part II---Utilizing Molecular Modeling Techniques to Study the Src-Interacting Proteins Actin Filament Associated Protein of 110 kDa (AFAP-110) and Cortactin. [D] . Jett, John Edward, Jr. 2012

机译：第一部分-评价低聚物形成对细胞色素P450 2C9电子转移和药物代谢的影响，第二部分-利用分子建模技术研究Src相互作用蛋白肌动蛋白丝相关蛋白110 kDa（AFAP-110）和Cortactin 。
6. Regioselective Covalent Immobilization of Recombinant Antibody Binding Proteins A G and Protein L for Construction of Antibody Arrays [O] . Jin-soo Seo, Sungwon Lee, C. Dale Poulter -1

机译：重组抗体结合蛋白AG和L的区域选择性共价固定化以构建抗体阵列
7. A network-based analysis of polyanion-binding proteins utilizing human protein arrays [O] . Salamat-Miller, N., Fang, J., Seidel, C., 2007

机译：利用人蛋白质阵列进行基于网络的聚阴离子结合蛋白分析

Construction of a Two-Dimensional Protein Array Utilizing a Cytochrome c555-Based Protein

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