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首页> 外文会议>International Workshop on Bioinformatics, Biochemistry, Biomedical Sciences >Effects of Adventitious Buds Regeneration from Three Kinds of Explants in Jatropha curcas
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Effects of Adventitious Buds Regeneration from Three Kinds of Explants in Jatropha curcas

机译：不定芽再生从三种外植体在麻风树凝乳中的影响

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An efficient method has been established to induce adventitious buds regeneration from 3 kinds of explants (hypocotyl, cotyledon leaf and leaf) of Jatropha curcas L. All the explants were treated by high concentrations (5-120 mg/L) of TDZ solution for different soak period (5-80 min) with the regeneration rate significantly enhanced and the quality of the regenerated buds dramatically improved. The best results of adventitious bud's regeneration were detected when explants were treated with 20 mg/L TDZ solution for 20 min before being inoculated on basic MS medium free of hormone for 30 days. What's more, addition of 0.4 mg/L GA_3 into the elongation medium was the most conducive to the extension of adventitious buds. By applying this method described in our paper, regeneration effect of J. curcas was remarkably improved.
机译：已经建立了一种有效的方法，以诱导来自Jatropha Curcas L的3种外科植体（下丘脑，子叶叶和叶）的不定芽再生。所有的外植体都是通过高浓度（5-120mg / L）的TDZ溶液处理的再生率浸泡（5-80分钟）显着提高，再生芽的质量显着改善。当使用20mg / L TDZ溶液处理20mg / L TDZ溶液20分钟后，检测到不定芽再生的最佳结果在接种在碱性MS培养基上没有激素30天。更重要的是，在伸长型介质中加入0.4 mg / l Ga_3是最有利于延伸不定芽的延伸。通过应用本文中描述的方法，J. Curcas的再生效果显着改善。

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