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Contamination of Malting Barley by Toxigenic Fungi of Genera Fusarium and Cochliobolus

机译:Weala Fusarium和Cochliobolus的毒性真菌污染麦芽大麦

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Nowadays investigations of safety assessment of foods might be carried out using nanotechnologies. Apart from medical diagnostics, PCR is now used in investigations of genetics analysis, discovering of genetically modified products and identification of microbial contaminants of food material. Contamination of malting barley by toxigenic microorganisms is of immediate interest for brewing industry. In this work we investigated grains of Russia-specific malting barley Benthe and Cherio of 2015 and 2016 harvesting years. "Germination abilities" of Benthe and Cherio seeds of the crop of 2015 and 2016 were low: 65.8% and 76%; 62.8% and 78%, respectively. Starch content in grains conforms to standards regardless the harvesting year and protein content of Bente of 2016 exceeds the regulated characteristics. Phytopathogenic microbial contamination of grain is mainly presented by fungi of Fusarium genus and Cochliobolus sativus species, but correlation between these phytopathogenic fungi varies depending on the harvesting year. Using species-specific primers in PCR we defined belonging of fungi of Fusarium genus to toxigenic Fusarium graminearum species for three Benthe samples of the 2016 harvesting year and for nine Cherio samples of the 2016 harvesting year.
机译:如今,可以使用纳米技术进行食物的安全评估的调查。除了医疗诊断外,PCR现在用于研究遗传分析,发现遗传修饰的产品和食品材料微生物污染物的鉴定。通过毒性微生物污染麦芽大麦是对酿造行业的立即兴趣。在这项工作中,我们调查了俄罗斯特定麦芽大麦的谷物,2015年和2016年的Cherio收获年。本河及2015年作物的Cherio种子的“发芽能力”低:65.8%和76%;分别为62.8%和78%。谷物中的淀粉含量符合标准,无论2016年百蚁的收获年份和蛋白质含量超过规定的特征。谷物的植物致病微生物污染主要由镰刀菌属和雪氏毒蕈族族族种类的真菌呈现,但这些植物致病性真菌之间的相关性根据收获年度而变化。在PCR中使用特异性底漆我们定义了2016年收获年份的三个Benthe样本和2016年收获年份的九个Cherio样本的镰刀菌属的镰刀菌属的镰刀菌属的真菌属性。

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