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首页> 外文会议>Universitas Riau International Conference on Science and Environment >Effect of LED Wavelengths and Light-Dark Cycle on Photosynthetic Production of Chlorella Kessleri for AlgaeBased Biosensor Optimization
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Effect of LED Wavelengths and Light-Dark Cycle on Photosynthetic Production of Chlorella Kessleri for AlgaeBased Biosensor Optimization

机译：LED波长和浅黑循环对藻类生物传感器优化小黄皮肤生成的影响

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摘要

Observation of cell culture with a micro sensor system for biomedical application in measuring chemical, biological and physical parameters are widely used to evaluate cell metabolism respiration. Cellular respiration depicts the energy formation and molecules in the form of dissolved oxygen (pO2) level needed in the photosynthesis process. This paper presents the observation of pO2 level produced from the algae chlorella kessleri influenced by LED light source at wavelength of 480 nm (blue) and 650 nm (red). It aims to show the relationship between photosynthesis production activities to the signals produced by each condition for biosensor optimization. Artificial light settings consist of light-dark cycle to simulate a day light period in producing chlorophyll and night period as a time of relaxation. Sample of 150μL chlorella kessleri with density 1.4·10~6 cell/ml is immobilized in biochip-C and stimulate with two difference wavelength light sources for 30 minutes period ON/OFF. The result shows that the red light has 18% pO2 level higher than blue light since the algae is sensitive to the red light and absorbed more energy to produce chlorophyll.
机译：用微传感器系统观察细胞培养用于测量化学，生物学和物理参数的生物医学应用，广泛用于评估细胞代谢呼吸。细胞呼吸描绘了光合作用过程中所需溶解的氧（PO2）水平的能量形成和分子。本文介绍了由LED光源受到480nm（蓝色）和650nm（红色）影响的LED光源影响的PO2水平的观察。它旨在显示光合作用生产活动与生物传感器优化的每个条件产生的信号之间的关系。人造光设置包括光暗循环，以模拟在叶绿素和夜间作为放松时间产生叶绿素和夜间的日光。具有密度1.4·10〜6个细胞/ ml的150μl小球藻kessleri的样品在Biochip-C中固定，并用两个差异波长光源刺激30分钟的ON / OFF。结果表明，由于藻类对红光敏感，红光具有高于蓝光的18％PO2水平，并且吸收更多的能量以产生叶绿素。

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