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Cre-mediated transgene integration into a pre-determined nuclear genomic site of Chlamydomonas reinhardtii

机译：Cre-介导的转基因整合到衣原体骨髓瘤的预定核基因组遗址中

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Chlamydomonas reinhardtii is a unicellular green alga that has attracted interest due to its potential biotechnological applications, such as algal biofuel and therapeutic protein production. Despite all the advantages that this unicellular alga offers, the generation of useful transgenic algae strains has been the lack of effective molecular tools. Especially, the overall poor expression of heterologous genes from nuclear genome remains an obstacle for basic and applied research. The purpose of this study is to generate transgenic microalgae capable of long-term stable expression by site-specific gene integration based on Cre/loxP system.

机译：Chlamydomonas Reinhardtii是一种独一无二的绿藻，由于其潜在的生物技术应用，如藻类生物燃料和治疗蛋白质生产，引起了利息。尽管这种单细胞藻类提供了所有优势，但有用的转基因藻类菌株的产生是缺乏有效的分子工具。特别是，来自核基因组的异源基因的总体表达仍然是基本和应用研究的障碍。本研究的目的是通过基于CRE / LOXP系统的位点特异性基因集成，产生能够长期稳定表达的转基因微藻。

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《化学工学会秋季大会》|2018年|140 p.|共1页
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Huang Guan; Kawabe Yoshinori; Ito Akira;
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中图分类化学工业;
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专利

1. 核基质结合区在转基因烟草中对转基因表达的影响 [J] . 李旭刚, 路子显, 陈蕾, 植物学报（英文版） . 2001,第004期
2. Validated Nuclear-Based Transgene Expression Regulated by the Fea1 Iron-Responsive Promoter in the Green Alga Chlamydomonas reinhardtii [J] . Coutinho Paula Barjona do Nascimento, Friedl Christine, Heilmann Marcus, Molecular biotechnology . 2019,第5期

机译：在绿藻骨藻藻藻藻藻藻藻藻藻藻藻藻藻藻藻糖尿病患者验证核基转基因表达。
3. Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit [J] . Lauersen Kyle J., Kruse Olaf, Mussgnug Jan H. Applied Microbiology and Biotechnology . 2015,第8期

机译：多功能模块化载体工具包在莱茵衣藻中靶向表达核转基因
4. Efficient recombinant protein production and secretion from nuclear transgenes in Chlamydomonas reinhardtii [J] . Lauersen Kyle J., Berger Hanna, Mussgnug Jan H., Journal of Biotechnology . 2013,第2期

机译：莱茵衣藻中高效重组蛋白的生产和核转基因的分泌
5. Cre-mediated transgene integration into a pre-determined nuclear genomic site of Chlamydomonas reinhardtii [C] . Huang Guan, Kawabe Yoshinori, Ito Akira 化学工学会秋季大会 . 2018

机译：Cre-介导的转基因整合到衣原体骨髓瘤的预定核基因组遗址中
6. Making Chlamydomonas reinhardtii a better model organism: Tackling the inefficiency of nuclear transgene expression and improving methods for the generation and characterization of insertional mutant libraries. [D] . Plucinak, Thomas M. 2013

机译：使莱茵衣藻成为更好的模型生物：解决核转基因表达的低效率问题，并改进插入突变体文库的生成和表征方法。
7. The Integration of Multiple Nuclear-Encoded Transgenes in the Green Alga Chlamydomonas reinhardtii Results in Higher Transcription Levels [O] . Noam Shahar, Shira Landman, Iddo Weiner, 2019

机译：绿藻衣原体Reinhardtii中多种核编码转基因的整合导致更高的转录水平
8. The Integration of Multiple Nuclear-Encoded Transgenes in the Green Alga Chlamydomonas reinhardtii Results in Higher Transcription Levels [O] . Noam Shahar, Shira Landman, Iddo Weiner, 2020

机译：绿藻衣原体Reinhardtii中多种核编码转基因的整合导致更高的转录水平

Cre-mediated transgene integration into a pre-determined nuclear genomic site of Chlamydomonas reinhardtii

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