首页> 外文会议>International seafood conference on the occasion of anniversary of the WEFTA >Effect of freezing and formaldehyde on solubility of natural actomyosin isolated from cod (gadus morhua), hake (merluccius merluccius) and blue whiting (micromesistius poutassou)
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Effect of freezing and formaldehyde on solubility of natural actomyosin isolated from cod (gadus morhua), hake (merluccius merluccius) and blue whiting (micromesistius poutassou)

机译:冷冻和甲醛对鳕鱼(GADUS MORHUA),HAKE(MERLUCCIUS MERLUCCIUS)和蓝色WHINGS(MicroMesistius Poutassou)分离的天然肌动酶溶解度的影响

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The decrease in solubility and the aggregates formed in natural actomyosin (NAM) isolated from cod, hake and blue whiting muscle and in NAM with 6mM formaldehyde (FA) added was studied in order to ascertain the way FA acts on myofibrillar proteins in FA forming species during frozen storage. Solubility in 0.6 M NaCl was measured before and after freezing (-20 deg C) and insoluble aggregates generated in frozen storage were treated with 2 percent sodium dodecyl sulphate (SDS) and 2 percent SDS+5 percent beta-mercaptoethanol (ME)_ successively in order to break down secondary interactions and disculfide bridges.
机译:研究了从COD,Hake和Bluit肌肉中分离的天然Actomyosin(NAM)中形成的溶解度和聚集体的降低,并添加了Nam,其中添加了6mM甲醛(FA),以确定FA在FA形成物种中的Myofibrillar蛋白上的方法 在冷冻储存期间。 在冻融(-20℃)之前和之后测量0.6M NaCl中的溶解度,并在冷冻储存中产生的不溶性聚集体用2%十二烷基硫酸钠(SDS)和2%SDS + 5%β-巯基乙醇(ME)_连续处理 为了分解次要相互作用和差异桥梁。

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