首页> 外文会议>International Grassland Congress >Development isolation and characterisation of a new non-virulent mimosine-degrading Klebsiella pneumoniae strain from the rumen liquor of German steers by using IBT-Goettinger bioreactor
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Development isolation and characterisation of a new non-virulent mimosine-degrading Klebsiella pneumoniae strain from the rumen liquor of German steers by using IBT-Goettinger bioreactor

机译:使用IBT-Goettinger生物反应器,从德国操纵器瘤胃瘤芽的新型非毒力咪喹蛋白降解的Klebsiella肺炎肺炎的显影分离与表征

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The tropical legume Leucaena leucocephala (leucaena) has many uses, including: a potential source of firewood and timber; for soil erosion control (Dijkman 1950); to provide shade; to enhance soil fertility; and as a nutritious forage for animal feed(Ruskin 1977). It is widely used as forage for cattle in tropical agriculture (Shclton 1998). In Myanmar, leucaena is used as a protein source in urea-molasses multi-nutrient blocks for ruminants (Ni Ni Maw elal. 2004). However, the use of leucaena as ruminant feed is not without problems, because it contains mimosine, a toxic anti-nutritional factor limiting its use as animal feed. Jones (1981) reported the absence of toxicity when leucaena was fed to goats and cattle in Hawaii and Indonesia. Accordingto the low dihydroxypyridine (DHP) in urine of those animals, it was assumed that they could degrade mimosine and DHP. Hawaiian goats, but not Australian goats, could degrade 3,4-DHP ruminally (Jones and Megarrity 1983). Inoculation of susceptible animals with rumen liquor containing mimosine-degrading bacteria protected against DHP toxicity in ruminants (Jones and Lowry 1984). For maintaining mimosine-degrading bacteria, the donor animals should be fed on leucaena continuously and it is expensive to maintain their veterinary care. Hence, we tried to develop mimosine-degrading ruminal bacteria using a fcrmcnter, intending to produce a source of inoculum for the routine control of leucaena toxicosis in ruminants.
机译:热带豆类leucaena leucocephala(leucaena)有很多用途,包括:柴火和木材的潜在来源;用于土壤侵蚀控制(Dijkman 1950);提供阴影;提高土壤肥力;作为动物饲料的营养饲料(Ruskin 1977)。它被广泛用作热带农业的牛(Shclton 1998)。在缅甸,leucaena用作反刍动物的尿素 - 糖蜜多营养块的蛋白质来源(Ni Ni Maw El.2004)。然而,使用Leucaena作为反刍动物饲料并非没有问题,因为它含有Mimosine,一种有毒的抗营养因子限制了其用作动物饲料。琼斯(1981)在夏威夷和印度尼西亚喂养山羊和牛的时候,据报道,缺乏毒性。根据这些动物的尿液中的低二羟基吡啶(DHP),假设它们可以降解Mimosine和DHP。夏威夷山羊,但不是澳大利亚山羊,可能会降级3,4-DHP(Jones和Mugarrity 1983)。用含有Mimosine降解细菌的瘤胃液接种易感动物,防止反刍动物的DHP毒性(Jones和Lowry 1984)。为了维持莫斯汀降解细菌,供体动物应不断喂食Leucaena,保持兽医护理是昂贵的。因此,我们试图使用FcRMCNTER开发Mimosine降解的瘤胃细菌,意图在反刍动物中产生常规控制Leucaena毒物的常规控制。

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