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首页> 外文会议>Ahmad Dahlan International Conference Series on Pharmacy and Health Science >Isolation of Actinomycetes from Sugarcane (Saccharum Officinarum) Rhizosphere and the Ability to Produce Antibiotic
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Isolation of Actinomycetes from Sugarcane (Saccharum Officinarum) Rhizosphere and the Ability to Produce Antibiotic

机译：甘蔗（Saccharum OfficinArum）根际分离放线菌和生产抗生素的能力

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Actinomycetes are well-known microorganism as secondary metabolites producers which serve as antibiotics. In this study, sugar cane (Saccharum officinarum) rhizosphere soil samples were collected from Piyungan, Bantul, Yogyakarta. The aims of this study were to obtain Actinomycetes isolates from sugar cane (Saccharum officinarum) rhizosphere which potentially produce antibiotics and to analyse the Thin Layer Chromatography (TLC) profile of ethyl acetate extract of active Actinomycetes isolates. The method used in primary screening to evaluate Actinomycetes antibacterial activity from the sugar cane rhizosphere are agar block method and cup-plate method, and the extract activity assay used Kirby-Bauer method. Determination of antibiotic activity was observed by measuring the diameter of inhibition zone. Extract component was analyzed by Thin Layer Chromatography (TLC). The results of this research was found 39 isolates of Actinomycetes classified into 7 groups based on color grouping. The result of agar block test was revealed that among 39 isolates, four isolates (10.25%) were able to inhibit Staphylococcus aureus. They were Te.234, Te.235, Te.236, and Te.325 isolates. Inhibition zone of crude extracts from these isolates against Staphylococcus aureus were Te.234=7.67 mm, Te.235=3.75 mm, Te.236=12.42 mm, and Te.325=19.16 mm. However, none of these isolates were able to inhibit Escherichia coli. Results of TLC profile showed that the suspected compounds contained in ethyl acetate extract of culture broth are alkaloid, polypeptide, steroid, alcohol, phenol and ketone/aldehyde compounds. Based on the results, it can be concluded that Actinomycetes can be isolated from sugar cane rhizosphere which has potential as an antibiotic producer by narrow spectrum to inhibit Gram-positive bacteria such as Staphylococcus aureus.
机译：作为次级代谢物生产商，放线菌是一种辅助的微生物，其作为抗生素。在这项研究中，从Piyungan，Bantul，Yogyakarta收集了甘蔗（Saccharum OfficinArum）根际土壤样品。本研究的目的是获得来自甘蔗（Saccharum OfficinArum）根际的放线菌分离株，其潜在地产生抗生素并分析活性放线菌的乙酸乙酯提取物的薄层色谱（TLC）谱。在初级筛选中用于评估来自甘蔗根际的抗菌活性的初级筛选的方法是琼脂块方法和杯板方法，提取活性测定使用Kirby-Bauer方法。通过测量抑制区的直径观察抗生素活性的测定。通过薄层色谱法（TLC）分析提取组分。发现该研究的结果39分离为基于颜色分组的7组分类的分离物。琼脂嵌段试验的结果显示，在39个分离物中，四个分离物（10.25％）能够抑制金黄色葡萄球菌。它们是TE.234，TE.235，TE.236和TE.325分离物。来自金黄色葡萄球菌的这些分离株的粗提取物的抑制区是TE.234 = 7.67mm，TE.235 = 3.75mm，TE.236 = 12.42mm，TE.325 = 19.16mm。然而，这些分离物中没有任何抑制大肠杆菌。 TLC型材的结果表明，培养肉汤乙酸乙酯提取物中含有的疑似化合物是生物碱，多肽，类固醇，醇，苯酚和酮/醛化合物。基于结果，可以得出结论，放线菌可以从甘蔗根圈中分离，其具有窄光谱作为抗生素生产商的潜力，以抑制革兰氏阳性细菌，例如金黄色葡萄球菌。

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