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首页> 外文会议>International Conference and Congress of the Entomological Society of Indonesia >Molecular Confirmation of Forensic Important Flies Collected in Palembang, South Sumatra
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Molecular Confirmation of Forensic Important Flies Collected in Palembang, South Sumatra

机译：在南苏达拉省帕莱蒙宫收集的法医学重要苍蝇的分子确认

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The identification of insect species is an important key in forensic entomology. Molecular examination of the nucleotide sequences of cytochrome c oxidase subunit II (COII) gene has been widely performed before the morphology identification. We analyzed the partial conserved COII nucleotide sequence of the forensic related flies. After the DNA extraction from collected larvae, thus PCR amplification of conserved sequencing of the partial COII sequence was implemented. Obtained sequences were analyzed for a phylogenetic tree and a distance matrix. Our data showed a very low intraspecific sequence distance among Calliphoridae and Sarcophagidae to the flies" species of Chrysomyia megacephala. Further research should attempt to determine the larval growth of newly discovered flies, to apply the time of death in forensic crime setting.
机译：昆虫物种的鉴定是法医昆虫学的重要关键。 在形态学鉴定之前广泛进行了细胞色素C氧化酶亚基II（COII）基因的核苷酸序列的分子检查。 我们分析了法医相关苍蝇的部分保守COII核苷酸序列。 在来自收集的幼虫的DNA提取后，实施了部分COII序列的保守测序的PCR扩增。 分析了系统发育树和距离基质的获得序列。 我们的数据在熊群岛和嗜酸酵母梅西米亚群岛的苍蝇中呈现出非常低的内部内序距离。进一步的研究应该试图确定新发现的苍蝇的幼虫生长，以申请法医犯罪环境中的死亡时间。

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