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首页> 外文会议>International Conference and Congress of the Entomological Society of Indonesia >Biochemical Characterization of Acetamiprid Resistance in Laboratory-Bred Population of Aedes aegypti L. Larvae
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Biochemical Characterization of Acetamiprid Resistance in Laboratory-Bred Population of Aedes aegypti L. Larvae

机译：亚艾氏血红蛋白植物植物抗乙醛抗性的生化特征

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摘要

The constant rise in cases of Zika, Dengue and Chikungunya worldwide has made control of Aedes aegypti a principal concern. The most recommended plan to control mosquito-borne diseases primarily lies on vector management and disturbing their disease-transmission cycle. Wide-ranging use of different classes of organic insecticides for mosquito control has led to the development of high levels of resistance making them less operative at safe dosages imposing us to explore novel insecticides. Present study investigates the bio-efficacy of a neonicotinoid, acetamiprid on the Ae. aegypti larvae, development of resistance after subjecting acetamiprid selection pressure for 10 successive generations and biochemical characterization of the resistance developed. Acetamiprid exposure of the parent population of Ae. aegypti early fourth instars resulted in respective LC_(50) and LC_(90) values of 0.188 ppm and 1.315 ppm. Selection with acetamiprid for 10 successive generations (ACSF-10) reduced its efficacy by 20-fold. Involvement of four enzymes; alpha-esterases, beta-esterases, glutathione-S-transferases and acetylcholinesterases in development of acetamiprid resistance was investigated to uncover mode of action of acetamiprid. An elevation of 1.4-fold and 2.1-fold was observed in alpha-esterases and beta-esterases activity in ACSF-10 as compared to ACSF-5. However, activity of glutathione-S-transferases decreased in ACSF-5 which rose to 12-fold in ACSF-10. Similarly, the activity of acetylcholinesterases was found to be much higher in resistant generations as compared to the parental strains. The results indicated individual/synergistic contribution of different enzymes leading to acetamiprid detoxification. Further research is being conducted to identify the role of target site mutations in resistance development.
机译：Zika，Dengue和Chikungunya案件的不断增加，使河东Aegypti控制了主要问题。控制蚊虫疾病的最推荐计划主要是向量管理和扰乱其疾病传输周期。广泛使用不同类别的有机杀虫剂用于蚊虫控制导致了高水平的抗性的发展，使其在安全剂量下施加我们探索新的杀虫剂。目前的研究研究了Neonicotinoid，AE上acetamiprid的生物疗效。 AEGYPTI幼虫，抗性后抗性的抗性，在acetamiprid选择压力下进行10种连续几种，抗性的生化表征。 AE父母群的acetamiprid暴露。 Aegypti早期的第四仪器导致相应的LC_（50）和LC_（90）值为0.188ppm和1.315ppm。用acetamiprid选择10个连续几代（ACSF-10）将其效率降低20倍。参与四种酶;研究了α-酯酶，β-酯酶，谷胱甘肽-S-转移酶和乙酰胆碱酯酶在发育乙基哌啶抗性的情况下，以发现acetamiprid的作用方式。与ACSF-5相比，在ACSF-10中以α-酯酶和β-酯酶活性观察到1.4倍和2.1倍的升高。然而，谷胱甘肽-S-转移酶的活性在ACSF-5中降低，在ACSF-10中升至12倍。类似地，与亲本菌株相比，发现乙酰胆碱酯酶的活性在抗性几代内较高。结果表明，不同酶的个体/协同贡献导致acetamiprid解毒。正在进行进一步的研究以确定靶位突变在抗性发育中的作用。

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