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首页> 外文会议>International conference on Asia-Pacific conference on science and management of coastal environment >Environmental andnutritional factors which regulate populationdynamics and toxin production in the dinoflagellate Alexandrium catenella
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Environmental andnutritional factors which regulate populationdynamics and toxin production in the dinoflagellate Alexandrium catenella

机译：调节人群体和毒素生产在Dinoflagellate Alexandrium catenella的环境和营养因素

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The effects of environmental and nutritional factors on population dynamics and toxin proudction were examined in Alexandrium catenella, maintained in enriched K media in laboratory cultures. Starting with a density of 50 cell ml~-1, the dinoflagellate population typically showed a lag phase and an exponential growth phase which lasted 14 days each, and then entered the stationary phase, with a maximal capacity of 12-18,000 cell ml~-1. Population densitites showed distinct diurnal patterns, with population growth beginning 2-4 hours in darkness. The optimal physical conditions for growth were pH 8.5, salinity of 30-35 per thousand, temperature of 20-25 deg c, and photoperiod of 14//10D to 16L/8D. The cell cycle was determiend by flow cytometry on synchronized batch cultures maintained at optimal pH, salinity, temperature and under 5 different photoperiod regimes. It was found that the G_1 phase was tmed to end at approximately 3 h after onset of darkness, and the G_2/M phase had begun at 4 hours. Nutrient supply markedly affected population growth. Under optimal physical conditions, the optimal concentrations for macronutrients and micronutrients were: NH_4~+ 0.025-0.2mM, NO_3~- 0.22-8.83 mM, glycerophosphate 0.04-0.06 mM, silicate 0.1-0.54 mM; FeEDTA 0.07-0.11 mM; Co 0.1 #mu#M, Cu 0.005-0.04 #mu#M; Mn 0.22-7.2 #mu#M; Mo 0.03-0.6 #mu#M; Se 0.02-0.1 #mu#M; Zn 0.04-1.6 #mu#M; thiamin 0.075-6 #mu#M; vitamin B_12 0.0004-0.004 #mu#M; biotin 0.007-0.015 #mu#M; EDTA 5-40 #mu#M.
机译：在亚历山大尼托尼亚亚洲群岛的亚历山大尼亚菌检测了环境和营养因素对人口动态和毒素交流的影响。从密度为50个细胞m1〜-1，达氨基葡萄糖群通常显示出滞后阶段和指数生长阶段，其每次持续14天，然后进入固定相，最大容量为12-18,000个细胞m1- - 1。人口密度显示出不同的昼夜模式，人口增长在黑暗中开始2-4小时。生长的最佳物理条件是pH8.5，盐度为30-35％，温度为20-25℃，光周期为14 // 10d至16l / 8d。细胞周期通过流式细胞术在同步分批培养物上保持在最佳pH，盐度，温度和5次不同的光周期制度下的流式细胞术。发现在黑暗发生后，G_1相位在大约3小时内被TME末端，并且G_2 / M相在4小时内开始。营养供应显着影响人口增长。在最佳物理条件下，常规营养素和微量营养素的最佳浓度为：NH_4〜+ 0.025-0.2mm，NO_3〜 - 0.22-8.83mm，甘油磷酸0.04-0.06mm，硅酸盐0.1-0.54 mm; feedta 0.07-0.11 mm; CO 0.1＃MU＃M，CU 0.005-0.04＃mu＃m; Mn 0.22-7.2＃mu＃m; mo 0.03-0.6＃m; se 0.02-0.1＃mu＃m; Zn 0.04-1.6＃m;泰国0.075-6＃m;维生素B_12 0.0004-0.004＃mu＃m; Biotin 0.007-0.015＃mu＃m; EDTA 5-40＃mu＃m。

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