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首页> 外文会议>CBCME 2011;International Conference on Biotechnology, Chemical and Materials Engineering >Characterization of Codon Usage Bias in UL13 Gene of the Duck Plague Virus
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Characterization of Codon Usage Bias in UL13 Gene of the Duck Plague Virus

机译：鸭瘟病毒UL13基因密码子使用偏差的特征

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A comprehensive analysis of codon usage bias of DPV UL13 gene (GenBank Accession No. EU195098) was performed to provide a basis for understanding the relevant mechanism for its biased usage of synonymous codons and for selecting suitable expression systems to improve the expression of UL13 genes. Our study showed that codon usage bias of DPV UL13 gene strongly prefered to the synonymous with A and T at the third codon position. And ENC value and GC3s contents of the codon usage bias of UL13 gene in DPV were significantly different compared with those in other 21 reference herpesviruses. The phylogentic analysis about the putative protein of DPV UL13 and the 21 reference herpesviruses revealed that DPV was evolutionarily closer to the AnHV-1. In addition, the codon usage bias of DPV UL13 gene was compared with those of E. coli, yeast and human. There are 23 codons showing distinct usage differences between DPV and E. coli, 12 codons between DPV and yeast, 21 codons between DPV and human. Therefore, the yeast expression system is more appropriate for heterologous expression of the DPV UL13 gene.
机译：对DPV UL13基因的密码子使用偏差（GenBank登录号Eu195098）进行综合分析，为理解其偏见使用同义密码子的相关机制和选择合适的表达系统以改善UL13基因的表达来提供依据。我们的研究表明，DPV UL13基因的密码子使用偏差强烈优选在第三密码子位置的A和T中的同义。与其他21个参考疱疹病毒相比，DPV中的UL13基因的密码子使用偏差的CoCon使用偏差的含量和GC3S含量显着不同。关于DPV UL13的推定蛋白和21个参考疱疹病毒的Phylogentic分析显示DPV进化较近ANHV-1。此外，将DPV UL13基因的密码子使用偏差与大肠杆菌，酵母和人类的Codon使用偏差。有23个密码子，显示DPV和大肠杆菌之间的不同使用差异，DPV和酵母之间的12个密码子，DPV和人之间的21个密码子。因此，酵母表达系统更适合于DPV UL13基因的异源表达。

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