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Electrochemical Microbial Sensor for Rapid Detection and Quantification of Total Bacterial Count

机译:电化学微生物传感器,用于快速检测和定量总细菌计数

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Early and online detection of pathogens in the agricultural sector is vital in terms of crop protection as well as economy. Within this context, an electrochemical microbial sensor with a locally formed electrocatalyst was used for rapid detection of gram-negative and gram-positive bacteria in a suspension of bacteria in water. Initially, the background signals of each bacterial species in water were determined. Following this, the current response from the sensor was used to detect total bacterial concentration of a suspension containing both types of bacteria. Escherichia coli and Staphylococcus aureus were used as the model bacteria for these experiments. These bacteria had the ability to hinder the reduction reaction occurring at the electrode surface, leading to a drop in the current. Concentrations ranging from 10~2 to 10~9 cfu/ml were detected by this sensor. By monitoring the total bacterial concentration of washed water online, this sensor could be of great use anywhere along the food chain, starting from crop production until the marketing of food.
机译:早期和在线检测农业部门的病原体在作物保护方面至关重要。在这种情况下,具有局部形成的电催化剂的电化学微生物传感器用于快速检测水中细菌悬浮液中的革兰氏阴性和革兰氏阳性细菌。最初,确定了每种细菌种类在水中的背景信号。在此之后,来自传感器的电流响应用于检测含有两种类型细菌的悬浮液的总细菌浓度。大肠杆菌和金黄色葡萄球菌用作这些实验的模型细菌。这些细菌具有阻碍在电极表面上发生的还原反应的能力,导致电流下降。通过该传感器检测到10〜2至10〜9 cfu / ml的浓度。通过在网上监测洗涤水的总细菌浓度,该传感器可能在食物链中的任何地方使用,从作物生产开始,直到营销食物。

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