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CHIRAL ANALYSIS OF AMINO ACIDS USING COMPOSITE BIENZYME BIOSENSORS

机译:复合双酶生物传感器手性分析氨基酸

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摘要

The construction and performance of composite graphite-Teflon electrodes, in which the enzymes L- or D-amino acid oxidase and peroxidase are coimmobilized, together with the mediator ferrocene, is described. This biosensor design allows a detection potential of 0.0 V to be applied. Optimization of experimental variables both in the batch and flow-injection modes is reported. Composite bienzyme electrodes show a good stability, and reproducibility, and their analytical characteristics are suitable to estimate the amino acid content in real samples.
机译:描述了复合石墨-特富龙电极的结构和性能,其中L-或D-氨基酸氧化酶和过氧化物酶与二茂铁介体一起固定化。这种生物传感器设计允许施加0.0 V的检测电势。报告了在批处理和流动注射模式下实验变量的优化。复合双酶电极显示出良好的稳定性和可重复性,其分析特性适合估算真实样品中的氨基酸含量。

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