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首页> 外文会议>Conference on reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications >In Vivo Imaging with Near-infrared Fluorescence Lifetime Contrast
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In Vivo Imaging with Near-infrared Fluorescence Lifetime Contrast

机译：体内成像与近红外荧光寿命对比度

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Fluorescence imaging is a mainstay of biomedical research, allowing detection of molecular events in both fixed and living cells, tissues and whole animals. Such high resolution fluorescence imaging is hampered by unwanted signal from intrinsic background fluorescence and scattered light. The signal to background ratio can be improved by using extrinsic contrast agents and greatly enhanced by multispectral imaging methods. Unfortunately, these methods are insufficient for deep tissue imaging where high contrast and speedy acquisition are necessary. Fluorescence lifetime (FLT) is an inherent characteristic of each fluorescent species that can be independent of intensity and spectral properties. Accordingly, FLT-based detection provides an additional contrast mechanism to optical measurements. This contrast is particularly important in the near-infrared (NIR) due to relative transparency of tissue as well as the broad absorption and emission spectra of dyes that are active in this region. Here we report comparative analysis of signal distribution of several NIR fluorescent polymethine dyes in living mice and their correlations with lifetimes obtained in vitro using solution models. The FLT data obtained from dyes dissolved in serum albumin solution correlated well with FLTs measured in vivo. Thus the albumin solution model could be used as a good predictive model for in vivo FLT behavior of newly developed fluorescent reporters. Subsequent experiments in vivo, including monitoring slow release kinetics and detecting proteinuria, demonstrate the complementary nature of FLT for fluorescence intensity imaging.
机译：荧光成像是生物医学研究的主干，允许在固定和活细胞，组织和整个动物中检测分子事件。这种高分辨率荧光成像由来自本征背景荧光和散射光的不需要的信号阻碍。通过使用外部造影剂并通过多光谱成像方法大大增强，可以提高到背景比的信号。不幸的是，这些方法对于深层组织成像不足，在那里需要高对比度和快速采集。荧光寿命（FLT）是每个荧光物质的固有特征，其可以与强度和光谱性能无关。因此，基于FLT的检测提供了对光学测量的附加对比机制。由于组织的相对透明度以及在该区域中活跃的染料的宽吸收和发射光谱，这种对比度在近红外（NIR）中尤为重要。在这里，我们报告了生物小鼠中几个NIR荧光聚甲藻染料的信号分布的比较分析及其使用溶液模型在体外获得的寿命的相关性。从溶解在血清白蛋白溶液中的染料中获得的FLT数据与体内测量的FLTS相关。因此，白蛋白溶液模型可以用作新开发的荧光报告者的体内FLT行为的良好预测模型。随后的体内实验，包括监测缓慢释放动力学和检测蛋白尿检测，证明了FLT的互补性，用于荧光强度成像。

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