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Localization and Tracking in 4D Fluorescence Microscopy Imagery

机译:4D荧光显微图像中的定位和跟踪

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3D fluorescence microscopy continues to pose challenging tasks with more experiments leading to identifying new physiological patterns in cells' life cycle and activity. It then falls on the hands of biologists to annotate this imagery which is laborious and time-consuming, especially with noisy images and hard to see and track patterns. Modeling of automation tasks that can handle depth-varying light conditions and noise, and other challenges inherent in 3D fluorescence microscopy often becomes complex and requires high processing power and memory. This paper presents an efficient methodology for the localization, classification, and tracking in fluorescence microscopy imagery by taking advantage of time sequential images in 4D data. We show the application of our proposed method on the challenging task of localizing and tracking microtubule fibers' bridge formation during the cell division of zebrafish embryos where we achieve 98% accuracy and 0.94 F1-score.
机译:3D荧光显微镜继续构成具有挑战性的任务,具有更多实验,导致识别细胞生命周期和活动中的新生理模式。然后它落在生物学家手中,向这个图像注释这一成像,这是一种费力且耗时的,特别是嘈杂的图像,难以看到和跟踪模式。自动化任务的建模,可以处理深度不同的光线条件和噪声,以及3D荧光显微镜中固有的其他挑战经常变得复杂,需要高处理电力和存储器。本文通过利用4D数据中的时间顺序图像介绍了荧光显微图像中的定位,分类和跟踪的有效方法。我们展示了我们提出的方法在斑马鱼胚胎细胞分裂过程中定位和跟踪微管纤维桥梁形成的挑战性任务,我们达到了98%的精度和0.94F1分数。

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