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Detection system of acid rain pollution using light-induced delayed fluorescence of plant leaf in vivo

机译:使用光诱导的植物叶片酸雨污染检测系统在体内植物叶的延迟荧光

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Photosynthetic apparatus is susceptible to environmental stress. Light-induced delayed fluorescence (DF) in plant is an intrinsic label of the efficiency of charge separation at P680 in photosystem II (PS II). In this investigation, we have developed a biosensor that can accurately inspect acid rain pollution by means of DF in vivo. Compared with traditional methods, the proposed technique can continuously monitor environmental changes, making fast, real-time and non-invasive inspection possible. The biosensor is an all-weather measuring instrument; it has its own illumination power and utilizes intrinsic DF as the measurement marker. With soybean (Glycine max (L.) Merr.) seedling as a testing model, which is sensitive to acid rain pollution, the relationship that delayed fluorescence properties and capability of photosynthetic apparatus after being affected by simulated acid rain with different pH value was studied. The current investigation has revealed that the changes of delayed fluorescence ( Φ_i=(DF_0-DF_s)/(DF_0)) can probably characterize the pollution degree of simulated acid rain. Inspecting the changes in DF characteristics (Φ_i) of plant leaf in vivo may be a new approach for the detection of acid rain pollution and its impact on the ecosystem.
机译:光合仪易受环境压力的影响。植物中光诱导的延迟荧光(DF)是照相系统II(PS II)中P680的电荷分离效率的内在标记。在这次调查中,我们开发了一种能够通过体内DF准确地检查酸雨污染的生物传感器。与传统方法相比,所提出的技术可以连续监测环境变化,使得快速,实时和非侵入性检查成为可能。生物传感器是全天候测量仪器;它具有自己的照明功率,并利用本征DF作为测量标记。用大豆(甘氨酸Max(L.)Merr。)幼苗作为对酸雨污染敏感的测试模型,研究了延迟荧光特性和光合液受到不同pH值的模拟酸雨后的荧光特性和能力的关系。目前的研究表明,延迟荧光的变化(φ_i=(df_0-df_s)/(df_0))可能表征模拟酸雨的污染程度。检查体内植物叶DF特性(φ_I)的变化可能是检测酸雨污染及其对生态系统的影响的新方法。

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