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首页> 外文会议>International Conference of Chinese Society of Micro-Nano Technology;Annual Conference of the Chinese Society of Micro-Nano Technology >A Porous Microfluidic Chip for Protein Extraction Based on Solid Phase Extraction Method
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A Porous Microfluidic Chip for Protein Extraction Based on Solid Phase Extraction Method

机译：基于固相萃取法的蛋白质提取多孔微流控芯片

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摘要

Based on the principle of solid phase extraction (SPE) and the special immunoreaction, a microfluidic chip integrated with porous matrix was developed for protein extraction. Porous matrix was achieved by electrochemical etching silicon in a HF/ethanol mixture, which was coated on the wall of the rectangular channel of the microchip to provide a surface-enlarging matrix. The surface morphology of the bare porous silicon and the porous silicon modified with the protein has been characterized by SEM. Non-porous chip and porous chip were used to extract protein. Compared with non-porous matrix, the porous matrix achieved higher extracted efficiency of protein. Then two methods of surface modification were employed on porous matrix for protein extraction. The surface modification with Protein A could extract more protein with less non-special absorption. Evaluation of the structure of the solid phase matrix and the surface modification process in the microfluidic chip, the porous microfluidic chip is able to be suitable for incorporation into micro total analysis system (μTAS).
机译：基于固相萃取（SPE）原理和特殊的免疫反应，开发了一种与多孔基质结合的微流控芯片，用于蛋白质的提取。多孔基体是通过在HF /乙醇混合物中对硅进行电化学刻蚀而获得的，该涂层被涂在微芯片矩形通道的壁上，以提供表面扩大的基体。裸露的多孔硅和用蛋白质修饰的多孔硅的表面形态已通过SEM进行了表征。使用无孔芯片和多孔芯片提取蛋白质。与无孔基质相比，多孔基质的蛋白质提取效率更高。然后在多孔基质上采用两种表面修饰方法进行蛋白质提取。蛋白A进行的表面修饰可以提取更多的蛋白质，而非特殊吸收更少。评估微流体芯片中的固相基质的结构和表面改性过程，多孔微流体芯片能够适合纳入微总分析系统（μTAS）中。

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