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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Microfluidic chips with reversed-phase monoliths for solid phase extraction and on-chip labeling
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Microfluidic chips with reversed-phase monoliths for solid phase extraction and on-chip labeling

机译：具有反相整料的微流控芯片，用于固相萃取和芯片上标记

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摘要

The integration of sample preparation methods into microfluidic devices provides automation necessary for achieving complete micro total analysis systems. We have developed a technique that combines on-chip sample enrichment with fluorescence labeling and purification. Polymer monoliths made from butyl methacrylate were fabricated in cyclic olefin copolymer microdevices and used for solid phase extraction. We studied the retention of fluorophores, amino acids and proteins on these columns. The retained samples were subsequently labeled with both Alexa Fluor 488 and Chromeo P503, and unreacted dye was rinsed off the column before sample elution. Additional purification was obtained from the differential retention of proteins and fluorescent labels. A linear relation between the eluted peak areas and concentrations of on-chip labeled heat shock protein 90 samples demonstrated the utility of this method for on-chip quantitation. Our fast and simple method of simultaneously concentrating and labeling samples on-chip is compatible with miniaturization and desirable for automated analysis.
机译：将样品制备方法集成到微流体设备中，可提供实现完整的微量总分析系统所需的自动化。我们开发了一种将片上样品富集与荧光标记和纯化相结合的技术。由甲基丙烯酸丁酯制成的聚合物整料在环状烯烃共聚物微器件中制备，并用于固相萃取。我们研究了在这些色谱柱上保留的荧光团，氨基酸和蛋白质。随后用Alexa Fluor 488和Chromeo P503标记保留的样品，并在洗脱样品之前将未反应的染料从色谱柱上冲洗掉。从蛋白质和荧光标记的差异保留中获得了额外的纯化。洗脱峰面积与芯片上标记的热激蛋白90样品浓度之间的线性关系表明，该方法可用于芯片上定量。我们在芯片上同时浓缩和标记样品的快速简便的方法与小型化兼容，是自动化分析的理想之选。

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