...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Analytical methods >On-chip fluorescent labeling using reversed-phase monoliths and microchip electrophoretic separations of selected preterm birth biomarkers
【24h】

On-chip fluorescent labeling using reversed-phase monoliths and microchip electrophoretic separations of selected preterm birth biomarkers

机译：使用反相整料和微芯片电泳分离所选早产生物标记物的芯片上荧光标记

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

On-chip preconcentration, purification, and fluorescent labeling are desirable sample preparation steps to achieve complete automation in integrated microfluidic systems. In this work, we developed electrokinetically operated microfluidic devices for solid-phase extraction and fluorescent labeling of preterm birth (PTB) biomarkers. Reversed-phase monoliths based on different acrylate monomers were photopolymerized in cyclic olefin copolymer microdevices and studied for the selective retention and elution of a fluorescent dye and PTB biomarkers. Octyl methacrylate-based monoliths with desirable retention and elution characteristics were chosen and used for on-chip fluorescent labeling of three PTB biomarkers. Purification of on-chip labeled samples was done by selective elution of unreacted dye prior to sample. Automated and rapid on-chip fluorescent labeling was achieved with similar efficiency to that obtained for samples labeled off chip. Additionally, protocols for microchip electrophoresis of several off-chip-labeled PTB biomarkers were demonstrated in poly(methyl methacrylate) microfluidic devices. This study is an important step toward the development of integrated on-chip labeling and separation microfluidic devices for PTB biomarkers.
机译：芯片上的预浓缩，纯化和荧光标记是在集成微流体系统中实现完全自动化的理想样品制备步骤。在这项工作中，我们开发了电动操作的微流控设备，用于固相提取和荧光标记早产（PTB）生物标志物。基于不同丙烯酸酯单体的反相整料在环烯烃共聚物微器件中进行光聚合，并研究了荧光染料和PTB生物标志物的选择性保留和洗脱。选择具有理想保留和洗脱特性的基于甲基丙烯酸辛酯的整料，并将其用于三种PTB生物标志物的芯片荧光标记。芯片上标记样品的纯化是通过在样品之前选择性洗脱未反应的染料来完成的。实现了自动化和快速的芯片上荧光标记，其效率与芯片外标记的样品相似。另外，在聚（甲基丙烯酸甲酯）微流体装置中证明了几种芯片外标记的PTB生物标记物的微芯片电泳方法。这项研究是开发用于PTB生物标记物的集成芯片上标记和分离微流体设备的重要一步。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号