• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Microscopy, Holography, and Interferometry in Biomedicine >Measurement of conformational states of Ca2+-ATPase in sarcoplasmic reticulum using phosphorescence anisotropy
【24h】

Measurement of conformational states of Ca2+-ATPase in sarcoplasmic reticulum using phosphorescence anisotropy

机译：磷光各向异性测量肌浆网中Ca2 + -ATPase的构象状态

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract: Ca$+2$PLU$/-activated adenosine triphosphatase isolated from rabbit muscle sarcoplasmic reticulum has been specifically labelled at a single lysine located at the putative ATP binding site with the triplet probe, eosin-5$PRM@-isothiocyanate. Labelled microsomes were suspended in buffer containing different cations to shift the enzyme to one or the other of its conformeric states, denoted E$-1$/ and E$-2$/. Samples in the different conformations were excited with a short laser pulse from a frequency doubled Nd:YAG laser. The time-resolved phosphorescence of the protein-bound probe was measured using a microcomputer-based dual-channel phosphorimeter over a temperature span of 2$DGR - 42$DGR C, and the emission anisotropy computed. The study suggests that in both conformeric states, the enzyme consists of different protein aggregates, however, the relative population of different protein aggregates may be different with the E$-2$/ form of the ATPase tending to form aggregates of larger size and the E$-1$/ form of the enzyme tending to form smaller aggregates. A more precise estimation of the sizes of the protein rotating species depends on accurate determination of the orientation of the label on the protein molecules. !24
机译：摘要：从兔肌肉肌质网中分离的Ca $ + 2 $ PLU $ /-活化的腺苷三磷酸酶已通过三联体探针曙红-5 $ PRM @-异硫氰酸酯专门标记在推定的ATP结合位点的单个赖氨酸上。将标记的微粒体悬浮在含有不同阳离子的缓冲液中，以使酶转变为构象状态的一种或另一种，表示为E $ -1 $和E $ -2 $ /。用倍频Nd：YAG激光的短激光脉冲激发不同构型的样品。使用基于微型计算机的双通道荧光计在2 $ DGR-42 $ DGR C的温度范围内测量蛋白结合探针的时间分辨磷光度，并计算发射各向异性。研究表明，在两种构象状态下，酶均由不同的蛋白质聚集体组成，但是，不同蛋白质聚集体的相对种群可能有所不同，ATP酶的E $ -2 $ /形式往往会形成较大的聚集体，并且E $ -1 $ /形式的酶倾向于形成较小的聚集体。蛋白质旋转物种大小的更精确估计取决于蛋白质分子上标记方向的准确确定。 ！24

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号