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Effects of Depleted Uranium on Soil Microbial Activity: A Bioassay Approach Using 14C-labeled Glucose

机译:贫铀对土壤微生物活性的影响:一种使用14C标记葡萄糖的生物测定方法

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The short and long term influence of depleted uranium (DU) on soil microbial populations remains largely understudied. To understand short term effect of DU on soil microbial activity, an incubation study was conducted using 14C-labeled glucose. Two soils of contrasting texture (Eurtic cambisol and Haplic podzol) were amended with increasing concentrations (0.5 mmol·L-1 to 10 mmol·L-1) of either potassium nitrate (KNO3) or DU as uranyl nitrate UO2(NO3)2. Following addition, 14C-labeled glucose was then added to the soil and 14CO2 production from the mineralization of glucose measured at different time intervals (1 h to 14 d) to assess microbial activity. Glucose mineralization by the microbial community showed non-significant effect by different concentrations of DU on both soils. Fitting a double first order kinetic equation revealed that 87%~92% of the added glucose was retained in the microbial biomass prior to mineralization. However, comparison of the kinetic values for different concentrations of KNO3 and DU also showed non-significant difference in both soils. The results imply that there is no significant deleterious effect of DU on soil microbial activity in the short (<24 h) or longer term (<30 d).
机译:贫铀(DU)对土壤微生物种群的短期和长期影响仍未得到充分研究。为了了解DU对土壤微生物活性的短期影响,使用14C标记的葡萄糖进行了孵育研究。用硝酸钾(KNO3)或DU(硝酸铀酰)UO2(NO3)2的浓度(0.5mmol·L-1至10mmol·L-1)增加浓度,对两种质地相反的土壤(Eurtic cambisol和Haplic podzol)进行了改良。添加后,将14 C标记的葡萄糖添加到土壤中,并在不同时间间隔(1 h至14 d)测量葡萄糖矿化产生的14CO 2,以评估微生物活性。微生物群落对葡萄糖的矿化作用在两种土壤上均显示出不同的DU浓度无显着影响。拟合一个一阶动力学方程表明,在矿化之前,微生物生物质中保留了87%〜92%的添加葡萄糖。但是,比较不同浓度的KNO3和DU的动力学值,在两种土壤中也没有显着差异。结果表明,在短期(<24 h)或长期(<30 d)内,DU对土壤微生物活性没有明显的有害作用。

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