首页> 外文会议>Multiphoton Microscopy in the Biomedical Sciences VI; Progress in Biomedical Optics and Imaging; vol.7 no.12 >Detection of Collagen by Second Harmonic Microscopy as a Diagnostic Tool for Liver Fibrosis
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Detection of Collagen by Second Harmonic Microscopy as a Diagnostic Tool for Liver Fibrosis

机译:通过二次谐波显微镜检测胶原蛋白作为肝纤维化的诊断工具

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摘要

Liver fibrosis has many causes, including hepatitis C, alcohol abuse, and non-alcoholic steatohepatitis. It is characterized by abnormal deposition of extracellular matrix proteins, mainly collagen. The deposition of these proteins results in impaired liver function caused by distortion of the hepatic architecture by fibrous scar tissue. The unique triple helix structure of collagen and high level of crystallinity make it very efficient for generating second harmonic signals. In this study we have set out to see if second harmonic imaging of collagen can be used as a non-biased quantitative tool for classification of fibrosis levels in liver biopsies and if it can detect early fibrosis formation not detected by current methods.
机译:肝纤维化有多种原因,包括丙型肝炎,酗酒和非酒精性脂肪性肝炎。其特征在于细胞外基质蛋白(主要是胶原蛋白)的异常沉积。这些蛋白质的沉积导致肝功能受损,这是由纤维性瘢痕组织引起的肝结构变形引起的。胶原蛋白独特的三螺旋结构和高结晶度使其非常有效地产生二次谐波信号。在这项研究中,我们已经开始研究胶原蛋白的二次谐波成像是否可以用作肝活检中纤维化水平分类的无偏定量工具,以及它是否可以检测当前方法未检测到的早期纤维化形成。

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