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首页> 外文会议>Multiphoton Microscopy in the Biomedical Sciences VII; Progress in Biomedical Optics and Imaging; vol.8 no.19; Proceedings of SPIE-The International Society for Optical Engineering; vol.6442 >Epi-detected Coherent anti-Stokes Raman Scattering Imaging of Deep Tissues in vivo
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Epi-detected Coherent anti-Stokes Raman Scattering Imaging of Deep Tissues in vivo

机译：Epi检测到的体内深层组织的相干抗斯托克斯拉曼散射成像

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摘要

We report in vivo molecular imaging of mouse sciatic nerve by epi-detected coherent anti-Stokes Raman Scattering (E-CARS) microscopy with vibrational selectivity, high signal-to-background ratio, 3D spatial resolution, and real-time imaging capability. The large CARS signal from the CH_2 stretch vibration allows highly sensitive and selective imaging of the myelin membrane which possesses a high lipid to protein ratio. The underlying contrast mechanism of in vivo CARS is explored by 3D imaging of fat cells that surround the nerve as well as dermal adipocytes in the mouse ear. Simultaneous E-CARS imaging of myelinated axons and second harmonic generation imaging of the surrounding collagen fibers were performed in vivo without any labeling. Finally, we show that CARS microscopy is able to distinguish between healthy myelin and disintegrated myelin induced by lysolecithin based on decrease in E-CARS intensity as well as loss of dependence on excitation polarization. Our system provides a multimodality in vivo imaging tool for studying neurodegenerative disorders.
机译：我们报告通过epi检测相干的反斯托克斯拉曼散射（E-CARS）显微镜对小鼠坐骨神经的体内分子成像，具有振动选择性，高信噪比，3D空间分辨率和实时成像能力。 CH_2拉伸振动产生的大CARS信号允许对髓磷脂膜进行高度灵敏和选择性的成像，该膜具有很高的脂蛋白比率。体内CARS的基本反差机制是通过3D成像成像的，脂肪细胞围绕着神经以及老鼠耳朵中的真皮脂肪细胞。在体内进行髓鞘轴突的同时E-CARS成像和周围胶原纤维的二次谐波成像，无需任何标记。最后，我们显示CARS显微镜能够基于E-CARS强度降低以及对激发极化的依赖性丧失，来区分健康髓磷脂和溶血卵磷脂诱导的崩解髓磷脂。我们的系统提供了一种用于研究神经退行性疾病的体内多模态成像工具。

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