• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Multiphoton Microscopy in the Biomedical Sciences VII; Progress in Biomedical Optics and Imaging; vol.8 no.19; Proceedings of SPIE-The International Society for Optical Engineering; vol.6442 >Combined spectrally-resolved multiphoton microscopy and transmission microscopy employing a high-sensitivity electron-multiplying CCD camera
【24h】

Combined spectrally-resolved multiphoton microscopy and transmission microscopy employing a high-sensitivity electron-multiplying CCD camera

机译：结合光谱分辨多光子显微镜和透射显微镜，采用高灵敏度电子倍增CCD相机

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have developed a fast, sensitive multiphoton microscope employing a tunable femtosecond laser and an electron-multiplying CCD camera (EMCCD) in a non-descanned detection scheme. In one configuration, the microscope provides "standard" multiphoton fluorescence images with a relatively high acquisition rate (limited only by the speed of the scanners) over a broad band of emission wavelengths. In a second configuration, the use of a diffractive optic element allows acquisition of one full set of spectrally-resolved images after only one complete scan. Increased speed is achieved in this configuration by setting the integration time of the camera such that an entire line is scanned at once, with the spectrum at each point being perpendicular to the scanned line. In both configurations, a suitable optical arrangement permits acquisition of high resolution images for both multiphoton excitation of fluorescence and wide-field transmission microscopy. To illustrate the performances of our microscope we present spectrally-resolved images of individual fluorescent molecules spread over a surface as well as fluorescence images of yeast cells expressing a membrane receptor tagged with a variant of the green fluorescent protein.
机译：我们已经开发了一种快速，灵敏的多光子显微镜，该显微镜在非反扫描检测方案中采用了可调飞秒激光和电子倍增CCD相机（EMCCD）。在一种配置中，显微镜在宽的发射波长带上提供具有相对较高的采集速率（仅受扫描仪的速度限制）的“标准”多光子荧光图像。在第二种配置中，衍射光学元件的使用允许仅在一个完整的扫描之后采集一组完整的光谱分辨图像。通过设置摄像机的积分时间，可以一次扫描整条线，并且每个点的光谱都垂直于扫描线，因此可以提高速度。在这两种配置中，合适的光学装置都可以获取高分辨率图像，以用于荧光的多光子激发和宽视野透射显微镜。为了说明我们的显微镜的性能，我们展示了散布在整个表面上的单个荧光分子的光谱分辨图像，以及表达带有绿色荧光蛋白变体标记的膜受体的酵母细胞的荧光图像。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号