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首页> 外文会议>Multiphoton microscopy in the biomedical sciences XIV >Tunable dual-wavelength 2-picosecond light source for coherent Raman scattering microscopy
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Tunable dual-wavelength 2-picosecond light source for coherent Raman scattering microscopy

机译：可调谐双波长2皮秒光源，用于相干拉曼散射显微镜

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In narrow-bandwidth coherent Raman scattering (CRS) microscopy, efficient signal generation is accomplished with two-color laser sources providing synchronized picosecond pulses whose frequency difference and spectral widths match the molecular Raman frequency and bandwidth, respectively. With vibrational bandwidths of typically 10 cm~(-1), the optimum laser pulse durations thus correspond to about 2 ps. Here, we present a new light source consisting of an amplified Yb-fiber oscillator providing 2-ps pulses at 1031 nm and a synchronously green-pumped optical parametric oscillator (OPO). The OPO slightly shortens the pulses to ＜ 2 ps while maintaining a bandwidth of 10 cm~(-1). Output power levels of 1 W in both the 1031-nm and the OPO-branch with continuously tunable frequency differences between the two beams covering a broad range from 700 to 4500 cm~(-1) are achieved. In addition to CARS microscopy, this light source allows for SRS imaging via an integrated electro-optical modulation of the 1031-nm beam at 20 MHz with a depth of ＞95%, locked to the laser repetition rate of 80 MHz. The OPO noise at 20 MHz was found to be only 60% above the combined detector and laser noise of a conventional Nd:YVO pump source. This represents a significant reduction in laser noise when compared to other fiber-based laser sources previously proposed for SRS microscopy. When SRS imaging with this new light is compared with a Nd:YVO pumped OPO (delivering 7 ps and 5 ps pulses, respectively), a 5- to 6-fold increase in SRS signal strength and signal-to-noise ratio has been achieved. Video-rate SRS and the capability of multi-spectral SRS imaging are demonstrated.
机译：在窄带宽相干拉曼散射（CRS）显微镜中，有效的信号生成是通过提供同步皮秒脉冲的双色激光源完成的，该皮秒脉冲的频率差和频谱宽度分别与分子拉曼频率和带宽相匹配。在典型的10cm-1（-1）的振动带宽下，最佳的激光脉冲持续时间因此对应于约2ps。在这里，我们提出了一种新的光源，它由一个在1031 nm处提供2 ps脉冲的放大Yb光纤振荡器和一个同步绿泵浦光参量振荡器（OPO）组成。 OPO将脉冲略微缩短至<2 ps，同时保持10 cm〜（-1）的带宽。在1031 nm和OPO分支中，输出功率均达到1 W，两个光束之间的频率差可连续可调，覆盖700至4500 cm〜（-1）的宽范围。除了CARS显微镜外，该光源还可以通过对2031 MHz处的1031 nm光束进行集成电光调制来进行SRS成像，深度> 95％，锁定到80 MHz的激光重复频率。发现20 MHz处的OPO噪声仅比常规Nd：YVO泵浦源的组合检测器和激光噪声高60％。与先前为SRS显微镜提出的其他基于光纤的激光源相比，这意味着激光噪声的显着降低。当使用这种新光对SRS成像与Nd：YVO泵浦的OPO（分别提供7 ps和5 ps脉冲）进行比较时，SRS信号强度和信噪比提高了5到6倍。演示了视频速率SRS和多光谱SRS成像的功能。

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