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首页> 外文会议>Multiphoton microscopy in the biomedical sciences XVII >Mapping the orientation of corneal suturai lamellae by means of backward-scattered SHG microscopy
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Mapping the orientation of corneal suturai lamellae by means of backward-scattered SHG microscopy

机译：通过向后散射SHG显微镜绘制角膜缝合线的方向图

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摘要

SHG microscopy was successfully used to characterize the orientation of suturai lamellae within corneal samples by means of an epi-detection scheme. In particular, the attention was focused on the organization and orientation of corneal collagen lamellae in the first 30 um of the stromal layer below Bowman's membrane. In fact, it is thought that the irregular orientation of these specific lamellae, an in particular the existence of so-called suturai lamellae, affects the mechanical properties of the cornea and is responsible of the overall corneal stiffness. The methodology was tested by acquiring image stacks of the central portion of the cornea in ex vivo samples of healthy corneas, keratoconic corneas and keratoconic corneas treated with cross-linking. These samples represent a good benchmark for testing the methodology, considering that keratoconus is an ophthalmic disease in which the cornea loses its stiffness and acquires an abnormal conical shape because of a different organization of suturai lamellae. The samples were first imaged and characterized on the basis of forward/backward SHG ratio, finding a different ratio in keratoconus with respect to both the healthy cornea and cross-linked keratoconus. The inclination of corneal suturai lamellae was then characterized by means of a three-dimensional correlation analysis on SHG images, acquired using a backward detection geometry. Such method provided good discrimination capabilities, demonstrating that this approach can be used not only for diagnosing keratoconus in a very early stage, but also for performing treatment follow-up on cross-linked corneas.
机译：SHG显微镜已成功地通过Epi检测方案表征了角膜样品中缝合线的方向。特别是，注意力集中在Bowman膜下方的前30 um基质层中角膜胶原薄片的组织和方向。实际上，据认为这些特定的薄片的不规则取向，特别是所谓的缝合线薄片的存在，影响了角膜的机械性能并且是整个角膜硬度的原因。通过获取经过交联处理的健康角膜，圆锥角膜和圆锥角膜的离体样品中角膜中央部分的图像堆栈来测试该方法。考虑到圆锥角膜是一种眼科疾病，其中由于缝合线状片的组织不同，角膜失去了刚度并获得了异常的圆锥形，因此这些样品代表了测试方法的良好基准。首先对样品进行成像并根据前向/后向SHG比率进行表征，发现圆锥角膜相对于健康角膜和交联圆锥角膜的比率不同。然后，通过对SHG图像进行三维相关分析，对角膜缝合线片的倾斜度进行表征，该图像使用后向检测几何图形获取。这种方法提供了良好的辨别能力，表明该方法不仅可以用于非常早期的圆锥角膜诊断，还可以用于对交联的角膜进行治疗随访。

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  • 会议地点 San Francisco(US)
  • 作者

    R. Mercatelli; F. Ratto; F. Rossi; L.Menabuoni; A. Malandrini; F. Tatini; R.Nicoletti; R. Pini; F.S. Pavone; R. Cicchi;

  • 作者单位

    National Institute of Optics, National Research Council (INO-CNR), Largo E. Fermi 6 - 50125,Florence, Italy;

    IFAC-CNR, Istituto di Fisica Applicata "N. Carrara", Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy;

    IFAC-CNR, Istituto di Fisica Applicata "N. Carrara", Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy;

    Department of Ophthalmology, Public Hospital of Prato, Piazza Ospedale 5, 59100 Prato (PO), Italy;

    Department of Ophthalmology, Public Hospital of Prato, Piazza Ospedale 5, 59100 Prato (PO), Italy;

    IFAC-CNR, Istituto di Fisica Applicata "N. Carrara", Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy;

    C.S.O S.r.l, Via degli Stagnacci 12/e, 50018 Scandicci (FI), Italy;

    IFAC-CNR, Istituto di Fisica Applicata "N. Carrara", Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy;

    European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Via Nello Carrara, 1 - 50019, Sesto Fiorentino, Italy;

    National Institute of Optics, National Research Council (INO-CNR), Largo E. Fermi 6 - 50125,Florence, Italy,European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Via Nello Carrara, 1 - 50019, Sesto Fiorentino, Italy;

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  • 正文语种 eng
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  • 关键词

    second-harmonic generation (SHG) microscopy; collagen, cornea; keratoconus;

    机译：次谐波产生（SHG）显微镜;胶原蛋白，角膜;圆锥角膜;

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