首页> 外文会议>Multiphoton microscopy in the biomedical sciences XVII >New approaches to intracellular drug imaging by stimulated Raman scattering microscopy
【24h】

New approaches to intracellular drug imaging by stimulated Raman scattering microscopy

机译:刺激拉曼散射显微镜对细胞内药物成像的新方法

获取原文
获取原文并翻译 | 示例

摘要

A range of proposed alkyne Raman tags are examined in-silico for activity and then synthesised generating a library of analogues of the natural product, anisomycin. We report the use of bisaryl butadiyne-anisomycin (BADY-anisomycin) in intracellular SRS microscopy studies of uptake and localisation within live and fixed cells. Following rational design and synthesis, BADY-anisomycin was shown to produce an intense Raman band at 2219 cm"1, that is centrally located within the cellular silent region and is approximately 60 times more Raman active than the corresponding propargyl-anisomycin. Finally, we demonstrate two-colour imaging utilising EdU, an alkyne-containing proliferation probe and BADY-anisomycin.
机译:在计算机上对一系列拟议的炔烃拉曼标签进行计算机活性检查,然后进行合成,生成天然产物茴香霉素类似物的文库。我们报告在活细胞和固定细胞内摄取和定位的细胞内SRS显微镜研究中使用了二芳基丁二炔-茴香霉素(BADY-茴香霉素)。经过合理的设计和合成,表明BADY-茴香霉素会在2219 cm“ 1处产生一条很强的拉曼光谱带,该条带位于细胞沉默区域的中央,比相应的炔丙基-茴香霉素的拉曼活性高约60倍。演示了利用含炔烃的增殖探针EdU和BADY-茴香霉素进行的双色成像。

著录项

  • 来源
  • 会议地点 San Francisco(US)
  • 作者单位

    Cancer Research UK Edinburgh Centre, Institute of Genetics and Molecular Medicine, The University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XR, U.K;

    Cancer Research UK Edinburgh Centre, Institute of Genetics and Molecular Medicine, The University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XR, U.K,EaStCHEM School of Chemistry, The University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh, EH9 3FJ, U.K.;

    Cancer Research UK Edinburgh Centre, Institute of Genetics and Molecular Medicine, The University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XR, U.K,Current address: Queens Medical Research Institute, The University of Edinburgh,47 Little France Crescent, Edinburgh, EH 16 4TJ, U.K.;

    EaStCHEM School of Chemistry, The University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh, EH9 3FJ, U.K.;

    Cancer Research UK Edinburgh Centre, Institute of Genetics and Molecular Medicine, The University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XR, U.K;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Anisomycin; SRS, Raman; SKBR3; EdU; Multimodal; JNK;

    机译:阿尼霉素SRS,拉曼; SKBR3;教育多式联运; JNK;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号