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首页> 外文会议>Multiphoton microscopy in the biomedical sciences XVII >Quantitative multi-parameter analysis of single molecule dynamics by PIE FastFLIM microscopy
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Quantitative multi-parameter analysis of single molecule dynamics by PIE FastFLIM microscopy

机译：通过PIE FastFLIM显微镜对单分子动力学进行定量多参数分析

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摘要

PIE FastFLIM microscopy allows the quantitative multi-parameter measurement of single molecule protein folding and dynamics. Using donor-acceptor FRET pair-labeled proteins, we detect changes in protein conformation and dynamics by monitoring FRET efficiency, stoichiometry and lifetime. Together with anisotropy decay information, we acquire rotational relaxation times for single molecules. By applying antibunching, FLCS and burst analysis, multi-parameters (such as copy numbers in protein complexes), diffusion coefficient and molecular brightness can be fitted for deeper understanding of the conformational dynamic behavior of single protein molecules. In this paper, we'll focus on the multiparameters of FRET efficiency, stoichiometry and lifetime.
机译：PIE FastFLIM显微镜可以对单分子蛋白质折叠和动力学进行定量多参数测量。使用供体-受体FRET对标记的蛋白质，我们通过监测FRET效率，化学计量和寿命来检测蛋白质构象和动力学的变化。连同各向异性衰减信息，我们获得了单个分子的旋转弛豫时间。通过应用反聚束，FLCS和猝发分析，可以拟合多参数（例如蛋白质复合物中的拷贝数），扩散系数和分子亮度，以更深入地了解单个蛋白质分子的构象动力学行为。在本文中，我们将重点关注FRET效率，化学计量和寿命的多参数。

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  • 会议地点 San Francisco(US)
  • 作者

    Yuansheng Sun; Ulas Coskun; Phoebe S. Tsoi; Josephine C. Ferreon; Allan Chris Ferreon; Beniamino Barbieri; Shih-Chu Jeff Liao;

  • 作者单位

    ISS, Inc., 1602 Newton drive, Champaign, IL 61822, USA;

    ISS, Inc., 1602 Newton drive, Champaign, IL 61822, USA;

    Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA;

    Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA;

    Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA;

    ISS, Inc., 1602 Newton drive, Champaign, IL 61822, USA;

    Author to whom correspondence should be addressed: Shih-Chu Jeff Liao, Ph.D., ISS Microscopy Group Leader. Address:1602 Newton drive, Champaign, IL 61822, USA;

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