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首页> 外文会议>Nanobiophotonics and Biomedical Applications III; Progress in Biomedical Optics and Imaging; vol.7, no.18 >Microfluidic Microwell and Microcapillary Biochips
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Microfluidic Microwell and Microcapillary Biochips

机译：微流控微孔和微毛细管生物芯片

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Utilizing nanotechnology, proprietary chemistry, and microfluidics, innovative firms are developing biochips and instrument systems that enable high-speed automated biomedical sequencing. Incom Inc. presents development results on five novel biochip technologies based on FiberOptic MicroSlide™ and microcapillary technology. FiberOptic MicroSlide™ are fiber optic interrogated (FOI) biochips made up of millions of fused optical fibers, and are uniquely suited as a platform for microarray applications. FiberOptic MicroSlide™henceforth referred to as "MicroSlides" or "slides" in this paper) act as a ''zero thickness substrate'' transmitting optical signals from top to bottom without spreading, so that fluorescent or luminescent activity on the surface or within a well can be directly coupled to a CCD device without additional optics. In contrast to bulk optics, the slides are compact and have excellent light-gathering power. They are an alternative to conventional microscope slides for applications involving moderate-resolution bottom viewing (inverted microscopy). The surface of the MicroSlides can be etched or patterned with a permanent polymer to form microwell arrays, or microfluidic structures suitable for genomic and proteomic analysis, cell migration studies and other applications. Low-cost microcapillary array plates have also been developed. These plates act as microscopic test tubes, which enable picoliter reactions to be detected, counted and analyzed. Progress in developing large area (300 mm X 300 mm) arrays with up to 100 million capillaries, and diameter / length aspect ratios up to 10,000: 1 is presented. Results demonstrate negligible optical cross talk between capillaries, resulting in improved signal-to-noise ratios while minimizing false hits.
机译：创新公司利用纳米技术，专有化学和微流体技术，开发生物芯片和仪器系统，以实现高速生物医学自动测序。 Incom Inc.展示了基于FiberOptic MicroSlide™和微毛细管技术的五种新型生物芯片技术的开发成果。 FiberOptic MicroSlide™是由数百万个融合光纤组成的光纤询问（FOI）生物芯片，非常适合用作微阵列应用程序的平台。因此，FiberOptic MicroSlide™（在本文中称为“ MicroSlides”或“ slides”）充当“零厚度基板”，从顶部到底部传输光信号而不会扩散，从而使表面或内部的荧光或发光活性该井可以直接耦合到CCD设备，而无需其他光学器件。与块状光学器件相比，这些滑块紧凑且具有出色的聚光能力。它们是常规显微镜载玻片的替代产品，适用于涉及中等分辨率底视（倒置显微镜）的应用。 MicroSlides的表面可以用永久性聚合物蚀刻或形成图案，以形成微孔阵列或微流体结构，适用于基因组和蛋白质组学分析，细胞迁移研究和其他应用。还开发了低成本的微毛细管阵列板。这些板充当微观试管，可检测，计数和分析皮微升反应。介绍了开发具有多达1亿个毛细血管，直径/长度长宽比高达10,000：1的大面积（300 mm X 300 mm）阵列的进展。结果表明，毛细管之间的光学串扰可忽略不计，从而改善了信噪比，同时最大程度地减少了误击。

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