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首页> 外文会议>Nanobiophotonics and Biomedical Applications III; Progress in Biomedical Optics and Imaging; vol.7, no.18 >Biophotonic studies on lipid membranes using Oblique Incidence Reflectivity Difference (OI-RD) ellipsometry
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Biophotonic studies on lipid membranes using Oblique Incidence Reflectivity Difference (OI-RD) ellipsometry

机译：使用斜入射反射率差（OI-RD）椭圆光度法对脂质膜进行生物光子研究

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摘要

In this study we report new results on the changes in optical properties of glass surfaces induced during wet photolithography using Oblique Incidence Reflectivity Difference Ellipsometry (OI-RD). A novel wet UV-photolithographic method for patterning phospholipid bilayers into two-dimensional arrays of voids and patches on hydrophilic glass substrates is presented. Especially, this technique involves etching the glass substrate fused with lipid vesicle solution and subsequently illuminated with short-wavelength UV light through a photo mask thus creating voids in the irradiated regions. The effects of the chemical etching and subsequent UV irradiation on the surfaces of microscope glass slides are investigated using the OI-RD technique. In this study, we have observed that the UV irradiation after chemical etching further changes the properties of the surface, even in the absence of the lipid bilayer. As a result, irradiating the chemically etched surface before the UV photolithography step renders the whole surface homogenous. Furthermore, fluorescence recovery after photobleaching (FRAP) experiments have been conducted on such homogenized surfaces which reveal that the fluidic properties of the membranes are retained. The created patterns are suitable to study protein-DNA interactions in the lipid environment. Our long term goal is to utilize this technique as a new screening approach for testing drug interactions above and below the cell surface.
机译：在这项研究中，我们报告了使用斜入射反射率差椭偏法（OI-RD）在湿法光刻过程中引起的玻璃表面光学特性变化的新结果。提出了一种新颖的湿式UV光刻方法，用于将磷脂双层图案化为亲水性玻璃基板上的空隙和斑块的二维阵列。特别地，该技术涉及蚀刻融合有脂质囊泡溶液的玻璃基板，随后通过光掩模用短波长紫外光照射，从而在照射区域中产生空隙。使用OI-RD技术研究了化学蚀刻和随后的紫外线辐射对显微镜载玻片表面的影响。在这项研究中，我们已经观察到，即使在没有脂质双层的情况下，化学蚀刻后的紫外线照射也会进一步改变表面的特性。结果，在UV光刻步骤之前辐照化学蚀刻的表面使整个表面均匀。此外，已经在这种均质化的表面上进行了光漂白（FRAP）实验后的荧光恢复，这揭示了膜的流体性质得以保留。创建的模式适用于研究脂质环境中蛋白质与DNA的相互作用。我们的长期目标是利用这项技术作为一种新的筛选方法，以测试细胞表面上方和下方的药物相互作用。

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