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Bright and compact macromolecular probes for bioimaging applications

机译：适用于生物成像应用的明亮而紧凑的高分子探针

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摘要

Amphiphilic macromolecules with multiple borondipyrromethene (BODIPY) chromophores appended to a common poly(methacrylate) backbone were synthesized by the random co-polymerization of appropriate methacrylate monomers. The resulting polymers incorporate also hydrophilic oligo(ethylene glycol) and hydrophobic decyl side chains designed to impose aqueous solubility and insulate the chromophoric components from each other respectively. The presence of multiple chromophores translates into a significant enhancement in molar absorption coefficient, relative to a model BODIPY monomer. The effective insulation of the fluorophores minimizes interchromophoric interactions and mitigates depressive effects on the fluorescence quantum yield. The overall result is a 6-fold enhancement in brightness, relative to the model monomer. These macromolecular probes can be injected into live Caenorhabditis elegans to allow their visualization with a 4-fold increase in signal intensity, relative to the model system. Furthermore, they can be conjugated to secondary antibodies, under standard amide-coupling conditions, with negligible influence on the binding affinity of the biomoleucles to allow the implementation of immunolabeling protocols.
机译：通过适当共聚的甲基丙烯酸酯单体的无规共聚反应，合成了具有多个硼二吡咯亚甲基（BODIPY）发色团的两亲大分子，该发色团附加在一个共同的聚（甲基丙烯酸酯）主链上。所得的聚合物还结合了亲水性的低聚（乙二醇）和疏水性的癸基侧链，其被设计成施加水溶性并分别使发色组分彼此绝缘。相对于模型BODIPY单体，多种发色团的存在转化为摩尔吸收系数的显着提高。荧光团的有效绝缘可最大程度地减少发色团之间的相互作用，并减轻对荧光量子产率的抑制作用。相对于模型单体，总体结果是亮度提高了6倍。可以将这些大分子探针注入活的秀丽隐杆线虫中，以使其信号强度相对于模型系统提高4倍。此外，它们可以在标准酰胺偶联条件下与二抗偶联，对生物分子的结合亲和力的影响可以忽略不计，从而可以实施免疫标记方案。

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  • 来源
  • 会议地点 San Francisco(US)
  • 作者

    Ek Raj Thapaliya; Yang Zhang; Pravat Dhakal; Adrienne S. Brown; James N. Wilson; Kevin M. Collins; Francisco M. Raymo;

  • 作者单位

    Laboratory for Molecular Photonics, Departments of Biology and Chemistry, 1301 Memorial Drive, University of Miami, Coral Gables, FL 33146-0431;

    Laboratory for Molecular Photonics, Departments of Biology and Chemistry, 1301 Memorial Drive, University of Miami, Coral Gables, FL 33146-0431;

    Laboratory for Molecular Photonics, Departments of Biology and Chemistry, 1301 Memorial Drive, University of Miami, Coral Gables, FL 33146-0431;

    Laboratory for Molecular Photonics, Departments of Biology and Chemistry, 1301 Memorial Drive, University of Miami, Coral Gables, FL 33146-0431;

    Laboratory for Molecular Photonics, Departments of Biology and Chemistry, 1301 Memorial Drive, University of Miami, Coral Gables, FL 33146-0431;

    Laboratory for Molecular Photonics, Departments of Biology and Chemistry, 1301 Memorial Drive, University of Miami, Coral Gables, FL 33146-0431;

    Laboratory for Molecular Photonics, Departments of Biology and Chemistry, 1301 Memorial Drive, University of Miami, Coral Gables, FL 33146-0431;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Amphiphilic polymers; BODIPYs; fluorescence imaging; immunolabeling; macromolecular probes; nanoparticles; self-assembly;

    机译：两亲性聚合物；身体;荧光成像免疫标记大分子探针纳米粒子自组装;

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