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Live cell imaging based on surface plasmon-enhanced fluorescence microscopy using random nanostructures

机译:基于使用随机纳米结构的表面等离激元增强型荧光显微镜的活细胞成像

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摘要

Localized surface plasmon enhanced microscopy based on nanoislands of random spatial distribution was demonstrated for imaging live cells and molecular interactions. Nanoislands were produced without lithography by high temperature annealing under various processing conditions. The localization of near-field distribution that is associated with localized surface plasmon on metallic random nanoislands was analyzed theoretically and experimentally in comparison with periodic nanostructures. For experimental validation in live cell imaging, mouse macrophage-like cell line stained with Alexa Fluor 488 was prepared on nanoislands. The results suggest the possibility of attaining the imaging resolution on the order of 80 nm.
机译:演示了基于随机空间分布的纳米岛的局部表面等离子体激元增强显微镜用于活细胞和分子相互作用的成像。通过在各种工艺条件下进行高温退火,无需光刻即可形成纳米岛。与周期性纳米结构相比,从理论和实验上分析了与金属随机纳米岛上的局部表面等离子体激元有关的近场分布的局部性。为了在活细胞成像中进行实验验证,在纳米岛上制备了用Alexa Fluor 488染色的小鼠巨噬细胞样细胞系。结果表明有可能获得约80 nm的成像分辨率。

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  • 来源
  • 会议地点 San Francisco CA(US)
  • 作者单位

    School of Electrical and Electronic Engineering, Yonsei University, Seoul, Korea 120-749;

    School of Electrical and Electronic Engineering, Yonsei University, Seoul, Korea 120-749;

    School of Electrical and Electronic Engineering, Yonsei University, Seoul, Korea 120-749;

    Institute for Immunology and Immunological Diseases and Severance Biomedical Science Institute, College of Medicine, Yonsei University, Seoul, Korea 120-752;

    Institute for Immunology and Immunological Diseases and Severance Biomedical Science Institute, College of Medicine, Yonsei University, Seoul, Korea 120-752;

    School of Electrical and Electronic Engineering, Yonsei University, Seoul, Korea 120-749;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Surface plasmon; Super-resolution image; Rigorous coupled-wave analysis; Nanoislands;

    机译:表面等离激元;超分辨率图像;严格的耦合波分析;纳米岛;

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