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首页> 外文会议>Nanoscale imaging, sensing, and actuation for biomedical applications XI >Amplification and modulation of fluorescent signals by using hybridization chain reactions for multiplexed sensing of biomolecules in a one-pot
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Amplification and modulation of fluorescent signals by using hybridization chain reactions for multiplexed sensing of biomolecules in a one-pot

机译：通过杂交链反应对一锅中生物分子进行多重传感的荧光信号放大和调制

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Fluorescence readout of molecular information is a promising approach for biomolecular sensing. For detection of enormous biomolecules via fluorescence, biomolecular information should be converted to codes that can be readout easily and simultaneously. For the purpose, we study a biomolecule fluorescence color (B/F) encoders that modulate fluorescence signals by control of fluorescence resonance energy transfer (FRET). The B/F encoder converts biomolecular signals into fluorescent color codes represented with fluorescent wavelengths and intensity levels. The combination offers a great number of codes for representing the biomolecular information. In this study, we discuss multiplexed detection of target biomolecules using B/F encoders. Use of the B/F encoders would offer a multiplexed biomolecular sensing in a one-pot without micro-fabrication like DNA microarray. In the experiments, we prepared B/F encoders based on two kinds of hybridization chain reactions (HCR) that make long double-stranded DNA polymers to control positions of fluorescence and quencher molecules. In the B/F encoders, target molecules trigger to start assembling the polymer structures. The fluorescent molecules in the absence of the targets are near the quenchers and the output fluorescence is suppressed by FRET. The polymerization process separates the fluorescent and quencher dyes and the fluorescent signal increase. The experimental results show that the B/F encoders based on HCRs have linear and independent response to each target, and temporal signals during the encoding reactions are usable for multiplexed readout. This result leads to the multiplexed sensing in a one-pot by fluorescent amplification and multiple fluorescent color-coding.
机译：分子信息的荧光读取是一种有前途的生物分子传感方法。为了通过荧光检测巨大的生物分子，应将生物分子信息转换为可以轻松同时读取的代码。为此，我们研究了一种通过控制荧光共振能量转移（FRET）来调制荧光信号的生物分子荧光颜色（B / F）编码器。 B / F编码器将生物分子信号转换为荧光色码，以荧光波长和强度水平表示。该组合提供了大量代表生物分子信息的代码。在这项研究中，我们讨论使用B / F编码器对目标生物分子的多重检测。 B / F编码器的使用将在一个锅中提供多重生物分子感测，而无需像DNA微阵列那样的微制造。在实验中，我们基于两种杂交链反应（HCR）制备了B / F编码器，该杂交反应使长的双链DNA聚合物能够控制荧光和淬灭分子的位置。在B / F编码器中，目标分子触发以开始组装聚合物结构。不存在靶标的荧光分子靠近淬灭剂，FRET抑制了输出荧光。聚合过程将荧光染料和猝灭染料分开，荧光信号增加。实验结果表明，基于HCR的B / F编码器对每个目标具有线性和独立的响应，并且在编码反应期间的时间信号可用于多路读取。该结果导致通过荧光放大和多种荧光颜色编码在一锅中的多路感测。

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