FLUORESCENCE ENERGY TRANSFER REAGENTS FOR DNA SEQUENCING AND ANALYSIS: High-throughput fluorescent DNA sequencing

机译：用于DNA测序和分析的荧光能量转移试剂：高通量荧光DNA测序

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摘要

A novel set of fluorescent reagents for DNA sequencing and analyses was developed using a fluorescence energy transfer principle with synthetic chemistry. These reagents are markedly superior when compared to single dye-labeled primers and dideoxynucleotides in DNA sequencing and in PCR-fragment analysis. Examples of construction, characterization, and application of these fluorescent reagents are described.

机译：利用荧光能量转移原理和合成化学技术，开发了一套用于DNA测序和分析的新型荧光试剂。与DNA测序和PCR片段分析中的单一染料标记的引物和双脱氧核苷酸相比，这些试剂具有明显的优越性。描述了这些荧光试剂的构造，表征和应用的实例。

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来源
《NATO Advanced Research Workshop on Biological, Biophysical amp; Theoretical Aspects of Polymer Structure and Transport Jun 20-25, 1999 Bikal, Hungary》|1999年|p.371-383|共13页
会议地点 Bikal(HU)
作者
Jingyue Ju;
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作者单位

Dept. of Chemical Engineering and the Columbia Genome Center, Columbia University, NY, NY 10032;

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会议组织
原文格式 PDF
正文语种 eng
中图分类一般工业技术;
关键词
fluorescence energy transfer; DNA sequencing; oligodeoxynucleotide; ET primer; dideoxynucleotide; fluorophore; donor; acceptor; absorption; emission; elec-trophoresis;

机译：荧光能量转移； DNA测序；寡脱氧核苷酸； ET引物；双脱氧核苷酸；荧光团；供体；受体；吸收；发射；电泳;

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专利

1. Two-step cycle sequencing improves base ambiguities and signal dropouts in DNA sequencing reactions using energy-transfer-based fluorescent dye terminators. [J] . Wen L Molecular biotechnology . 2001,第2期

机译：使用基于能量转移的荧光染料终止剂，两步循环测序可改善DNA测序反应中的碱基歧义和信号缺失。
2. Fluorescent dye-primer cycle sequencing using unpurified PCR products as templates; development of a protocol amenable to high-throughput DNA sequencing [J] . Karen M. Vossen, Michael G. Fried Nucleic Acids Research . 1995,第12期

机译：使用未纯化的PCR产物作为模板的荧光染料引物循环测序;开发适用于高通量DNA测序的方案
3. Fluorescent dye-primer cycle sequencing using unpurified PCR products as templates; development of a protocol amenable to high-throughput DNA sequencing [J] . Nucleic acids research . 1995,第12期

机译：使用未纯化的PCR产物作为模板的荧光染料引物循环测序;开发适用于高通量DNA测序的方案
4. FLUORESCENCE ENERGY TRANSFER REAGENTS FOR DNA SEQUENCING AND ANALYSIS: High-throughput fluorescent DNA sequencing [C] . Jingyue Juauthor_name/, NATO NATO advanced research workshop on biological, biophysical theoretical aspects of polymer structure and transport . 2002

机译：用于DNA测序和分析的荧光能量转移试剂：高通量荧光DNA测序
5. Transcriptomic analysis using high-throughput sequencing and DNA microarrays. [D] . Fox, Samuel E. 2011

机译：使用高通量测序和DNA芯片进行转录组分析。
6. Fluorescence energy transfer dye-labeled primers for DNA sequencing and analysis. [O] . J Ju, C Ruan, C W Fuller, 1995

机译：荧光能量转移染料标记的引物用于DNA测序和分析。
7. Fluorescence energy transfer dye-labeled primers for DNA sequencing and analysis. [O] . Ju, J, Ruan, C, Fuller, C W, 1995

机译：荧光能量转移染料标记的引物，用于DNA测序和分析。
8. High-throughput Analysis with 96-Capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection. [R] . Xue, G. 2001

机译：基于激光诱导荧光检测的96-毛细管阵列电泳和DNa序列整合样品制备的高通量分析。

FLUORESCENCE ENERGY TRANSFER REAGENTS FOR DNA SEQUENCING AND ANALYSIS: High-throughput fluorescent DNA sequencing

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