Towards improved applications of cell-free protein biosynthesis - the influence of mRNA structure and suppressor tRNAS on the efficiency of the system

机译：致力于改进无细胞蛋白质生物合成的应用-mRNA结构和抑制性tRNAS对系统效率的影响

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The cell-free protein biosynthesis has the potential to become a powerful technology for the biochemical research in particular in the determination of the structure and function of proteins. The number of possible applications is rising with the obtainable yields and with the expanded feasibility of introducing modified amino acids into proteins. Here we describe the influence of two RNA translation components, the mRNA and the suppressor tRNA, on the efficiency of protein biosynthesis.

机译：无细胞蛋白质生物合成具有成为生物化学研究的强大技术的潜力，特别是在蛋白质结构和功能的测定中。随着可获得的产量以及将修饰的氨基酸引入蛋白质的扩展的可行性，可能的应用数目正在增加。在这里我们描述了两个RNA翻译组件，mRNA和抑制tRNA，对蛋白质生物合成效率的影响。

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《NATO Advanced Research Workshop on RNA Biochemistry and Biotechnology Poznan, Poland October 10-17, 1998》|1998年|p.335-345|共11页
会议地点 Poznan(PL)
作者
Michael Gerrits; Helmut Merk; Wolfgang Stiege; Volker A. Erdmann;
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正文语种 eng
中图分类生物化学;
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专利

1. Properties of phenylalanine transfer ribonucleic acid with modified 3′-terminal end in protein biosynthesis using a rabbit reticulocyte cell-free system: effect of the replacement of cytidine residues from the CpCpA end of tRNA by 5-iodocytidine or 2-thiocytidine [J] . Abraham A. Hochberg, Alma Gal, Eli Keshet (Baksht), Nucleic acids research . 1977,第7期

机译：兔网织红细胞无细胞系统在蛋白质生物合成中具有修饰的3'-末端的苯丙氨酸转移核糖核酸的性质：用5-碘吡啶或2-硫代胞嘧啶置换tRNA的CpCpA末端的胞苷残基的作用
2. Translation of the mRNAs coding for the major hemolymph proteins of Ceratitis capitata in cell-free system: Comparison of the translatable mRNA levels to the respective biosynthetic levels of the proteins in the fat body during development [J] . A.C. Mintzas, G. Chrysanthis, C. Christodoulou, Developmental biology . 1983,第2期

机译：在无细胞系统中编码头孢状角膜炎主要血淋巴蛋白的mRNA的翻译：发育过程中脂肪体中可翻译的mRNA水平与相应蛋白质的生物合成水平的比较
3. A Concept for Selection of Codon-Suppressor tRNAs Based on Read-Through Ribosome Display in anIn VitroCompartmentalized Cell-Free Translation System [J] . AtsushiOgawa, MasayoshiHayami, ShinsukeSando, Journal of Nucleic Acids . 2012,第4期

机译：在体外隔室无细胞翻译系统中基于读取的核糖体展示选择密码子抑制性tRNA的概念。
4. Towards improved applications of cell-free protein biosynthesis - the influence of mRNA structure and suppressor tRNAS on the efficiency of the system [C] . Michael Gerrits, Helmut Merk, Wolfgang Stiege, NATO advanced research workshop on RNA biochemistry and biotechnology . 1999

机译：改进细胞蛋白生物合成的应用 - mRNA结构和抑制TrNA对系统效率的影响
5. Enhancing cell-free protein synthesis for improved yields and efficiency of incorporating selenomethionine, selenocysteine and fluorophenylalanine. [D] . Schulte, Jennifer Theresa. 2006

机译：增强无细胞蛋白质合成，以提高产量和掺入硒代蛋氨酸，硒代半胱氨酸和氟代苯丙氨酸的效率。
6. Properties of phenylalanine transfer ribonucleic acid with modified 3-terminal end in protein biosynthesis using a rabbit reticulocyte cell-free system: effect of the replacement of cytidine residues from the CpCpA end of tRNA by 5-iodocytidine or 2-thiocytidine. [O] . E K Baksht, A Gal, N de Groot, 1977

机译：使用兔网织红细胞无细胞系统的蛋白质生物合成中具有修饰的3-末端的苯丙氨酸转移核糖核酸的性质：用5-碘代吡啶或2-硫代胞苷置换tRNA的CpCpA末端的胞苷残基的作用。
7. Properties of phenylalanine transfer ribonucleic acid with modified 3'-terminal end in protein biosynthesis using a rabbit reticulocyte cell-free system: effect of the replacement of cytidine residues from the CpCpA end of tRNA by 5-iodocytidine or 2-thiocytidine. [O] . Baksht, E K, Gal, A, de Groot, N, 1977

机译：使用兔网织红细胞无细胞系统的蛋白质生物合成中具有修饰的3'-末端的苯丙氨酸转移核糖核酸的性质：用5-碘代吡啶或2-硫代胞苷置换tRNA的CpCpA末端的胞苷残基的作用。

Towards improved applications of cell-free protein biosynthesis - the influence of mRNA structure and suppressor tRNAS on the efficiency of the system

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