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首页> 外文会议>Navigating the Global Landscape for the New Composites >BIO-BASED CALCIUM CARBONATE NANOPARTICLES FOR DRUG DELIVERY APPLICATIONS
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BIO-BASED CALCIUM CARBONATE NANOPARTICLES FOR DRUG DELIVERY APPLICATIONS

机译：生物基碳酸钙纳米颗粒在药物输送中的应用

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Targeted delivery of a cytotoxic drug is beneficial to maximize the efficacy of the drug and tornreduce the side effects. Drug delivery systems are designed for the administration of drugs tornhumans or animals. Recently, nanoparticle-based drug delivery systems are being developed torncontrol the release of drugs in the body, to protect the drugs from enzymatic or chemicalrndegradation, and to attain organ- or tissue-targeted delivery. Studies have shown that calciumrncarbonate (CaCO_3) nanoparticles are highly porous, biocompatible, biodegradable, and have pHsensitivernproperties. Such desirable properties make CaCO_3 nanoparticles one of the bestrncandidates for biological drug delivery systems. In this project CaCO_3 nanoparticles, whichrnwere extracted from egg shells, were studied for their cytotoxicity and drug loading capacity.rnThe effects of the nanoparticles on cell viability were studied using Human Colon Cancerrn(SW480) and Human Dermal Fibroblast (HDF) cell lines using the Lactate dehydrogenase –rnCytotoxicity Assay Kit. The cells were propagated in tissue culture dishes and then seeded in 96-rnwell plates before the experiments. A stock suspension of CaCO_3 nanoparticles was prepared asrn100mg/ml in phosphate buffer saline solution, then serial dilutions were made to treat the cells.rnAfter initial rounds of testing, 2-fold serial dilutions of nanoparticle suspensions starting atrn5mg/ml were prepared for the experiments. Observation of the cells and particles interactionrnsuggested that high concentrations CaCO_3 around 39.0625 μg/ml physically overburden the cells,rnbut concentrations at or below 9.765626 μg/ml show insignificant cytotoxicity to the cells tested.rnAfter further testing, this was proved to not be true. The applications of some concentrations ofrnthe nanoparticles were cytotoxic, but not very cytotoxic below 156.25 μg/ml. Preliminary resultsrnshow that the nanoparticles were more cytotoxic to the SW480 cells than they were to the HDFrncells within the concentrations that were used.
机译：细胞毒性药物的靶向递送有利于最大程度地发挥药物的功效并减轻副作用。药物输送系统设计用于管理人类或动物的药物。近来，正在开发基于纳米颗粒的药物递送系统以控制药物在体内的释放，以保护药物免于酶促或化学降解，并实现靶向器官或组织的递送。研究表明碳酸氢钙（CaCO_3）纳米颗粒具有高度的多孔性，生物相容性，可生物降解性，并具有pH敏感特性。这种期望的性质使CaCO 3纳米颗粒成为生物药物递送系统的最佳候选者之一。在该项目中，研究了从蛋壳中提取的CaCO_3纳米颗粒的细胞毒性和载药量。使用人类结肠癌（SW480）和人类皮肤成纤维细胞（HDF）细胞系，研究了纳米颗粒对细胞生存力的影响。乳酸脱氢酶–细胞毒性测定试剂盒。细胞在组织培养皿中繁殖，然后在实验前接种在96孔板中。在磷酸盐缓冲液中以100mg / ml的浓度制备CaCO_3纳米粒子储备悬浮液，然后进行系列稀释以处理细胞。在最初几轮测试后，制备了以atr5mg / ml开始的2倍系列纳米悬浮液的稀释液。 。对细胞和颗粒相互作用的观察表明，大约39.0625μg/ ml的高浓度CaCO_3物理上使细胞超负荷，但浓度等于或低于9.765626μg/ ml的细胞对被测细胞没有明显的细胞毒性。进一步测试后，证明这是不正确的。低于156.25μg/ ml的某些浓度的纳米颗粒的应用具有细胞毒性，但不是很强的细胞毒性。初步结果显示，在所使用的浓度范围内，纳米颗粒对SW480细胞的毒性大于对HDFrn细胞的毒性。

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