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首页> 外文会议>Ophthalmic technologies XXVII >Probing superstructure of chicken corneal stroma by Fourier transform second harmonic generation microscopy
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Probing superstructure of chicken corneal stroma by Fourier transform second harmonic generation microscopy

机译：傅里叶变换二次谐波产生显微镜探测鸡角膜基质的超微结构

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The unique organization of the corneal stromal collagen is responsible for mechanical strength and optical clarity of the eye. However, factors and reasons on formation of the corneal stroma is still not fully understood. Second-harmonic generation (SHG) is a nonlinear second order optical process occurring in noncentrosymmetric systems with a large hyperpolarizability. Through the combination of the second harmonic generation (SHG) microcopy and optimized Fourier-transform analysis, mature chicken corneas are investigated to probe the depth-dependent collagen organization of the corneal stroma. Our results show that the anterior stroma behaves like a fan-like distribution of successively and counterclockwisely rotated fibrous lamellae for paired corneas from the same chicken. However, the posterior stroma maintains a non-rotating pattern while increasing in depth. Surprisingly, the thickness of the anterior stroma remains almost constant throughout the temporal-nasal direction, but the posterior stroma does not behave the same. Through quantitative analysis, the natural transition of the anterior and posterior stroma is also determined. These findings enhance our understanding of the collagen-rich tissue in the chicken cornea model. Moreover, the Fourier-transform-based modality, in combination with SHG microscopy, serves as a promising tool to determine collagen alignment in embryonic development, tissue engineering and corneal diseases.
机译：角膜基质胶原的独特组织负责眼睛的机械强度和光学清晰度。然而，仍未完全了解形成角膜基质的因素和原因。次谐波生成（SHG）是非线性的二阶光学过程，发生在具有大超极化性的非中心对称系统中。通过结合二次谐波（SHG）显微镜和优化的傅立叶变换分析，研究了成熟的鸡角膜，以探查角膜基质的深度依赖性胶原组织。我们的结果表明，对于来自同一只鸡的成对角膜，前基质的行为表现为扇形分布，依次和逆时针旋转纤维薄片。然而，后基质在深度增加的同时保持非旋转模式。令人惊讶的是，前间质的厚度在整个颞鼻方向上几乎保持恒定，但是后间质的行为却不同。通过定量分析，还确定了前，后基质的自然过渡。这些发现增强了我们对鸡角膜模型中富含胶原蛋白组织的了解。此外，基于傅立叶变换的模式与SHG显微镜相结合，可作为一种有前途的工具，用于确定胚胎发育，组织工程和角膜疾病中的胶原蛋白排列。

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