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首页> 外文会议>Conference on ophthalmic technologies XXVII >Probing superstructure of chicken corneal stroma by Fourier transform second harmonic generation microscopy
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Probing superstructure of chicken corneal stroma by Fourier transform second harmonic generation microscopy

机译：傅里叶变换二次谐波产生显微镜探测鸡角膜基质的超大

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The unique organization of the corneal stromal collagen is responsible for mechanical strength and optical clarity of the eye. However, factors and reasons on formation of the corneal stroma is still not fully understood. Second-harmonic generation (SHG) is a nonlinear second order optical process occurring in noncentrosymmetric systems with a large hyperpolarizability. Through the combination of the second harmonic generation (SHG) microcopy and optimized Fourier-transform analysis, mature chicken corneas are investigated to probe the depth-dependent collagen organization of the corneal stroma. Our results show that the anterior stroma behaves like a fan-like distribution of successively and counterclockwisely rotated fibrous lamellae for paired corneas from the same chicken. However, the posterior stroma maintains a non-rotating pattern while increasing in depth. Surprisingly, the thickness of the anterior stroma remains almost constant throughout the temporal-nasal direction, but the posterior stroma does not behave the same. Through quantitative analysis, the natural transition of the anterior and posterior stroma is also determined. These findings enhance our understanding of the collagen-rich tissue in the chicken cornea model. Moreover, the Fourier-transform-based modality, in combination with SHG microscopy, serves as a promising tool to determine collagen alignment in embryonic development, tissue engineering and corneal diseases.
机译：角膜基质胶原蛋白的独特组织负责眼睛的机械强度和光学透明度。然而，仍然没有完全理解形成角膜基质的因素和原因。二次谐波产生（SHG）是在具有大超极化性的非中心体对称系统中发生的非线性二阶光学过程。通过二次谐波产生（SHG）显微镜的组合和优化的傅里叶变换分析，研究了成熟的鸡角基体以探测角膜基质的深度依赖性胶原蛋白组织。我们的研究结果表明，前基质的表现类似于来自同一鸡肉的配对角膜的连续和逆旋转纤维薄片的扇形分布。然而，后部基质在深度增加时保持不旋转图案。令人惊讶的是，前基质的厚度在整个鼻鼻方向上几乎恒定，但后部基质并不表现相同。通过定量分析，还确定了前躯和后基质的自然转变。这些发现提高了我们对鸡角膜模型中富含胶原蛋白的组织的理解。此外，基于傅立叶变换的型号与SHG显微镜组合用作确定胚胎发育，组织工程和角膜疾病中的胶原蛋白对齐的有希望的工具。

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